Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17065 | 3' | -54.2 | NC_004333.2 | + | 13728 | 0.7 | 0.516198 |
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Target: 5'- uCCAUgcUCGUGCCGUUGAAGUCGa-- -3' miRNA: 3'- -GGUGaaGGCGUGGCAGCUUCAGCgau -5' |
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| 17065 | 3' | -54.2 | NC_004333.2 | + | 26288 | 0.7 | 0.505593 |
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Target: 5'- gCGCgcaCCGCGCCGUCGAugcgaaagccGGUCGaCg- -3' miRNA: 3'- gGUGaa-GGCGUGGCAGCU----------UCAGC-Gau -5' |
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| 17065 | 3' | -54.2 | NC_004333.2 | + | 11625 | 0.72 | 0.405583 |
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Target: 5'- gUCACUUCgCGCGCgaacgUGUCGAAGUagCGCUGc -3' miRNA: 3'- -GGUGAAG-GCGUG-----GCAGCUUCA--GCGAU- -5' |
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| 17065 | 3' | -54.2 | NC_004333.2 | + | 19412 | 0.72 | 0.375404 |
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Target: 5'- gCCGCgacCCGCGCgGUCGAcgcggcgcucgcgcAGUUGCUGg -3' miRNA: 3'- -GGUGaa-GGCGUGgCAGCU--------------UCAGCGAU- -5' |
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| 17065 | 3' | -54.2 | NC_004333.2 | + | 18730 | 1.09 | 0.001209 |
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Target: 5'- cCCACUUCCGCACCGUCGAAGUCGCUAu -3' miRNA: 3'- -GGUGAAGGCGUGGCAGCUUCAGCGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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