miRNA: 3'- gGUGaaGGCGuGGCAGCU------UCaGCGAU- -5'
miRNA: 3'- ggUGAAGGCGUGGCaGCuUCAgCGau -5'
miRNA: 3'- gGUGaa----GGCGUGgCAGCUu--CAGCGau -5'
miRNA: 3'- gGUGAagGCGUGGCa-GCuuCaGCGau -5'
miRNA: 3'- -GGUG-AAGGCGUGGCa--GcUUCaGCGAU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17065 | 3' | -54.2 | NC_004333.2 | + | 27344 | 0.67 | 0.670439 |
|
Target: 5'- gCGCggCCGC-CCGUCGGgcaggaAGcCGCUAc -3' miRNA: 3'- gGUGaaGGCGuGGCAGCU------UCaGCGAU- -5' |
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| 17065 | 3' | -54.2 | NC_004333.2 | + | 31500 | 0.67 | 0.670439 |
|
Target: 5'- -uGCUUCCGCACCG-CGcGGU-GCa- -3' miRNA: 3'- ggUGAAGGCGUGGCaGCuUCAgCGau -5' |
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| 17065 | 3' | -54.2 | NC_004333.2 | + | 45550 | 0.68 | 0.648198 |
|
Target: 5'- gCGCgaugaaCCGCGCgGUCGGucuGUCGCg- -3' miRNA: 3'- gGUGaa----GGCGUGgCAGCUu--CAGCGau -5' |
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| 17065 | 3' | -54.2 | NC_004333.2 | + | 35789 | 0.68 | 0.63705 |
|
Target: 5'- aCACUggCGCGCCGggCGggG-CGCg- -3' miRNA: 3'- gGUGAagGCGUGGCa-GCuuCaGCGau -5' |
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| 17065 | 3' | -54.2 | NC_004333.2 | + | 21776 | 0.66 | 0.735903 |
|
Target: 5'- cCCACuUUCCGCGCUGcagCcAAG-CGCUAu -3' miRNA: 3'- -GGUG-AAGGCGUGGCa--GcUUCaGCGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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