Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17066 | 5' | -47.9 | NC_004333.2 | + | 4539 | 0.68 | 0.930778 |
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Target: 5'- gGugGUGCaggccGGAcgcACGGGcCGUGAGCu -3' miRNA: 3'- -UugCAUGaau--CCU---UGUCCaGUACUCG- -5' |
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| 17066 | 5' | -47.9 | NC_004333.2 | + | 32305 | 0.68 | 0.918251 |
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Target: 5'- cGACGUGCguc--AGCAGGUCGgucacGAGCg -3' miRNA: 3'- -UUGCAUGaauccUUGUCCAGUa----CUCG- -5' |
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| 17066 | 5' | -47.9 | NC_004333.2 | + | 35610 | 0.7 | 0.817763 |
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Target: 5'- gGACGUACUguacgugcAGGAACAGGUgAUccGCc -3' miRNA: 3'- -UUGCAUGAa-------UCCUUGUCCAgUAcuCG- -5' |
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| 17066 | 5' | -47.9 | NC_004333.2 | + | 29685 | 0.74 | 0.638589 |
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Target: 5'- uACGUAuCUUAGGAGCAGGUaaagcuaAUGucaAGCa -3' miRNA: 3'- uUGCAU-GAAUCCUUGUCCAg------UAC---UCG- -5' |
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| 17066 | 5' | -47.9 | NC_004333.2 | + | 21044 | 0.76 | 0.521875 |
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Target: 5'- --aGUGCUgcuccugGGGAcucggGCAGGUUAUGGGCg -3' miRNA: 3'- uugCAUGAa------UCCU-----UGUCCAGUACUCG- -5' |
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| 17066 | 5' | -47.9 | NC_004333.2 | + | 18523 | 1.12 | 0.003015 |
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Target: 5'- cAACGUACUUAGGAACAGGUCAUGAGCg -3' miRNA: 3'- -UUGCAUGAAUCCUUGUCCAGUACUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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