miRNA: 3'- -GCaGCaGCU--AGCUGCAGUGUG-AGCag -5'
miRNA: 3'- -GCAGCag----CUAGCUGCAGugugAGCAG- -5'
miRNA: 3'- gcaGCAGCUaGCUGCAGUGugAGCAG- -5'
miRNA: 3'- gcaGCAGCUagCUGCAGUGUGAGCaG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17069 | 5' | -52.3 | NC_004333.2 | + | 8703 | 0.69 | 0.732365 |
|
Target: 5'- cCG-CGcCGAgcUCGACGUCGCGCaUCGg- -3' miRNA: 3'- -GCaGCaGCU--AGCUGCAGUGUG-AGCag -5' |
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| 17069 | 5' | -52.3 | NC_004333.2 | + | 7341 | 0.69 | 0.721651 |
|
Target: 5'- cCGUCGcgcagaGAUCGGCGUCuaa-UCGUCa -3' miRNA: 3'- -GCAGCag----CUAGCUGCAGugugAGCAG- -5' |
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| 17069 | 5' | -52.3 | NC_004333.2 | + | 3382 | 0.71 | 0.622521 |
|
Target: 5'- --aUGUCGAcCGGCGUCGCcgUCGUCg -3' miRNA: 3'- gcaGCAGCUaGCUGCAGUGugAGCAG- -5' |
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| 17069 | 5' | -52.3 | NC_004333.2 | + | 3035 | 0.69 | 0.699962 |
|
Target: 5'- --cCGUUGAcgGGCGUCACGCUCG-Cg -3' miRNA: 3'- gcaGCAGCUagCUGCAGUGUGAGCaG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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