Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17073 | 5' | -53.5 | NC_004333.2 | + | 30223 | 0.7 | 0.57627 |
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Target: 5'- gCCUGAUcGAUCGaCGcGCCGCgcgCGAUc -3' miRNA: 3'- -GGACUGaCUAGC-GCuUGGCGa--GCUGu -5' |
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| 17073 | 5' | -53.5 | NC_004333.2 | + | 45836 | 0.71 | 0.521989 |
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Target: 5'- aCCUGuuCUGGUCG-GuGCUGCUCGAUg -3' miRNA: 3'- -GGACu-GACUAGCgCuUGGCGAGCUGu -5' |
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| 17073 | 5' | -53.5 | NC_004333.2 | + | 47771 | 0.71 | 0.521989 |
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Target: 5'- uCUUGAUcaagGAUCGCGcgcucuACgCGCUCGGCAa -3' miRNA: 3'- -GGACUGa---CUAGCGCu-----UG-GCGAGCUGU- -5' |
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| 17073 | 5' | -53.5 | NC_004333.2 | + | 28585 | 0.71 | 0.500818 |
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Target: 5'- --cGAUcaUGuAUCGCGggUCGCUCGGCu -3' miRNA: 3'- ggaCUG--AC-UAGCGCuuGGCGAGCUGu -5' |
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| 17073 | 5' | -53.5 | NC_004333.2 | + | 8970 | 0.72 | 0.449678 |
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Target: 5'- --gGGCUgGAUCGUGGGCCGUgcgCGGCGg -3' miRNA: 3'- ggaCUGA-CUAGCGCUUGGCGa--GCUGU- -5' |
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| 17073 | 5' | -53.5 | NC_004333.2 | + | 24786 | 0.72 | 0.430038 |
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Target: 5'- uCCUGGauguaGGUUGCGGGCaCGUUCGGCAg -3' miRNA: 3'- -GGACUga---CUAGCGCUUG-GCGAGCUGU- -5' |
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| 17073 | 5' | -53.5 | NC_004333.2 | + | 30990 | 0.73 | 0.410911 |
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Target: 5'- cCCUG-CUG-UUGCGGGCUGCUCGcGCGg -3' miRNA: 3'- -GGACuGACuAGCGCUUGGCGAGC-UGU- -5' |
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| 17073 | 5' | -53.5 | NC_004333.2 | + | 5515 | 0.73 | 0.401548 |
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Target: 5'- --cGGCgcuGUUGCGGGCUGCUCGACGg -3' miRNA: 3'- ggaCUGac-UAGCGCUUGGCGAGCUGU- -5' |
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| 17073 | 5' | -53.5 | NC_004333.2 | + | 44428 | 0.79 | 0.18711 |
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Target: 5'- gCUGGCUGGUgcgUGCGAGgCGCUCGGCc -3' miRNA: 3'- gGACUGACUA---GCGCUUgGCGAGCUGu -5' |
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| 17073 | 5' | -53.5 | NC_004333.2 | + | 15370 | 1.1 | 0.001209 |
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Target: 5'- uCCUGACUGAUCGCGAACCGCUCGACAu -3' miRNA: 3'- -GGACUGACUAGCGCUUGGCGAGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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