miRNA: 3'- -CAGUcaACG--CCGUAAa--GGCCAGUUAU- -5'
miRNA: 3'- -CAGUCAACGCC--GUAA--AG-GCCAGUuau -5'
miRNA: 3'- -CAGUCAaCGCCGuaAAGG-CCAGUuau -5'
miRNA: 3'- caGUCaACGCCGuaaAGGCCAGUUau -5'
miRNA: 3'- -CAGUCAACGCCGuAAAGGCCAGUUAU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17076 | 5' | -49.8 | NC_004333.2 | + | 7123 | 0.66 | 0.908244 |
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Target: 5'- cGUCG--UGCaaGGCGUUcaaCCGGUCGAUAg -3' miRNA: 3'- -CAGUcaACG--CCGUAAa--GGCCAGUUAU- -5' |
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| 17076 | 5' | -49.8 | NC_004333.2 | + | 25759 | 0.67 | 0.885884 |
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Target: 5'- gGUCGGUUGCGGgaCGUUggUCgUGGUCAu-- -3' miRNA: 3'- -CAGUCAACGCC--GUAA--AG-GCCAGUuau -5' |
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| 17076 | 5' | -49.8 | NC_004333.2 | + | 23871 | 0.7 | 0.739419 |
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Target: 5'- gGUCGGUcGCGGCugUUCCaGGUCGc-- -3' miRNA: 3'- -CAGUCAaCGCCGuaAAGG-CCAGUuau -5' |
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| 17076 | 5' | -49.8 | NC_004333.2 | + | 23241 | 0.72 | 0.600269 |
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Target: 5'- -gCGGgUGCGGCuucUCCGGUCAGg- -3' miRNA: 3'- caGUCaACGCCGuaaAGGCCAGUUau -5' |
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| 17076 | 5' | -49.8 | NC_004333.2 | + | 12962 | 0.97 | 0.015801 |
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Target: 5'- uGUCAGUUGCGGC-UUUCCGGUCAAUAg -3' miRNA: 3'- -CAGUCAACGCCGuAAAGGCCAGUUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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