Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17079 | 3' | -52.7 | NC_004333.2 | + | 19129 | 0.66 | 0.774805 |
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Target: 5'- cGGCGcgaUCGUggGCGGCgcGGUGCUCGc -3' miRNA: 3'- uCCGUa--AGCGa-UGCUGguUCACGAGC- -5' |
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| 17079 | 3' | -52.7 | NC_004333.2 | + | 30041 | 0.66 | 0.795216 |
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Target: 5'- uGGCGUUCGUcuUGAUgccGUGCUCGa -3' miRNA: 3'- uCCGUAAGCGauGCUGguuCACGAGC- -5' |
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| 17079 | 3' | -52.7 | NC_004333.2 | + | 35411 | 0.66 | 0.795216 |
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Target: 5'- aAGGCGagaUUCGCgucuggUACGACUAcGUGCgcgCGu -3' miRNA: 3'- -UCCGU---AAGCG------AUGCUGGUuCACGa--GC- -5' |
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| 17079 | 3' | -52.7 | NC_004333.2 | + | 6705 | 0.66 | 0.814901 |
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Target: 5'- gGGcGCAUUgGCgacgagccaUGCGugCAAcGUGCUCa -3' miRNA: 3'- -UC-CGUAAgCG---------AUGCugGUU-CACGAGc -5' |
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| 17079 | 3' | -52.7 | NC_004333.2 | + | 10678 | 0.66 | 0.824442 |
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Target: 5'- uGGCcgUgGCcgGCGGCCcauUGCUCGa -3' miRNA: 3'- uCCGuaAgCGa-UGCUGGuucACGAGC- -5' |
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| 17079 | 3' | -52.7 | NC_004333.2 | + | 42017 | 0.66 | 0.824442 |
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Target: 5'- -cGCGUgaagCGC-GCGGCCAAcgagcggggcGUGCUCGc -3' miRNA: 3'- ucCGUAa---GCGaUGCUGGUU----------CACGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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