Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17079 | 3' | -52.7 | NC_004333.2 | + | 39225 | 0.69 | 0.653996 |
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Target: 5'- cGGCcga-GCUGCGugCAA-UGCUCGa -3' miRNA: 3'- uCCGuaagCGAUGCugGUUcACGAGC- -5' |
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| 17079 | 3' | -52.7 | NC_004333.2 | + | 42017 | 0.66 | 0.824442 |
|
Target: 5'- -cGCGUgaagCGC-GCGGCCAAcgagcggggcGUGCUCGc -3' miRNA: 3'- ucCGUAa---GCGaUGCUGGUU----------CACGAGC- -5' |
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| 17079 | 3' | -52.7 | NC_004333.2 | + | 44421 | 0.68 | 0.686755 |
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Target: 5'- cGGGC--UCGCUggcugguGCGugCGAGgcGCUCGg -3' miRNA: 3'- -UCCGuaAGCGA-------UGCugGUUCa-CGAGC- -5' |
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| 17079 | 3' | -52.7 | NC_004333.2 | + | 45835 | 0.68 | 0.699074 |
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Target: 5'- -aGCug-CGCaACGGCCGcacGGUGCUCGc -3' miRNA: 3'- ucCGuaaGCGaUGCUGGU---UCACGAGC- -5' |
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| 17079 | 3' | -52.7 | NC_004333.2 | + | 47840 | 0.68 | 0.699074 |
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Target: 5'- cGGGCAUUucgggCGgUACGGCCAuuccGGUGCa-- -3' miRNA: 3'- -UCCGUAA-----GCgAUGCUGGU----UCACGagc -5' |
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| 17079 | 3' | -52.7 | NC_004333.2 | + | 48001 | 0.67 | 0.743041 |
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Target: 5'- cGGGCAguggacUCGCgccagGCG-CCGAucguGUGCUCGc -3' miRNA: 3'- -UCCGUa-----AGCGa----UGCuGGUU----CACGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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