Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17079 | 3' | -52.7 | NC_004333.2 | + | 10477 | 0.68 | 0.699074 |
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Target: 5'- cAGGaCAUUCGCUACuACCugaacGGcaucuUGCUCGa -3' miRNA: 3'- -UCC-GUAAGCGAUGcUGGu----UC-----ACGAGC- -5' |
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| 17079 | 3' | -52.7 | NC_004333.2 | + | 9490 | 0.68 | 0.710201 |
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Target: 5'- cGGGaauUUCaGCgcgGCGGCCAGcUGCUCGa -3' miRNA: 3'- -UCCgu-AAG-CGa--UGCUGGUUcACGAGC- -5' |
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| 17079 | 3' | -52.7 | NC_004333.2 | + | 9007 | 0.68 | 0.687878 |
|
Target: 5'- cGGCGUUCGCgGCuuGCCAGGcggcgcGCUCGc -3' miRNA: 3'- uCCGUAAGCGaUGc-UGGUUCa-----CGAGC- -5' |
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| 17079 | 3' | -52.7 | NC_004333.2 | + | 7387 | 0.67 | 0.752699 |
|
Target: 5'- gGGGCGagCGacaugacgcgugcCUGCGGCCAGGUcugcgcgaugcGCUCGa -3' miRNA: 3'- -UCCGUaaGC-------------GAUGCUGGUUCA-----------CGAGC- -5' |
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| 17079 | 3' | -52.7 | NC_004333.2 | + | 6705 | 0.66 | 0.814901 |
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Target: 5'- gGGcGCAUUgGCgacgagccaUGCGugCAAcGUGCUCa -3' miRNA: 3'- -UC-CGUAAgCG---------AUGCugGUU-CACGAGc -5' |
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| 17079 | 3' | -52.7 | NC_004333.2 | + | 405 | 0.7 | 0.585941 |
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Target: 5'- cAGGUAUUCGagcGCGGCgAuGUGCUUGg -3' miRNA: 3'- -UCCGUAAGCga-UGCUGgUuCACGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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