miRNA: 3'- -CGCUaCGCugC---GGGCCGc-UGaUGAGC- -5'
miRNA: 3'- -CGCUa-----CGCUGCGG--GCCGCuGAUGagc -5'
miRNA: 3'- -CGCUa--CGCUGCGg-GcCGCUGAUGAGC- -5'
miRNA: 3'- -CGCUACGC-UGCGGGCCG----CuGAUG---AGC- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17080 | 3' | -57.5 | NC_004333.2 | + | 45972 | 0.68 | 0.465468 |
|
Target: 5'- gGCGAgGCGugGuguCCCGGCcuGCcACUCGg -3' miRNA: 3'- -CGCUaCGCugC---GGGCCGc-UGaUGAGC- -5' |
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| 17080 | 3' | -57.5 | NC_004333.2 | + | 46994 | 0.67 | 0.536736 |
|
Target: 5'- cGCGAggcuucGCGGCGCUcgCGGCG-CUGCa-- -3' miRNA: 3'- -CGCUa-----CGCUGCGG--GCCGCuGAUGagc -5' |
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| 17080 | 3' | -57.5 | NC_004333.2 | + | 47486 | 0.66 | 0.589869 |
|
Target: 5'- gGCGAgcgGCGAUGUagCuGCGAUUGCUUGc -3' miRNA: 3'- -CGCUa--CGCUGCGg-GcCGCUGAUGAGC- -5' |
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| 17080 | 3' | -57.5 | NC_004333.2 | + | 48174 | 0.72 | 0.259471 |
|
Target: 5'- cGCGGUGCGcGCGCCCGGUugccGcCUGCgccUCGu -3' miRNA: 3'- -CGCUACGC-UGCGGGCCG----CuGAUG---AGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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