miRNA: 3'- cGCuaCGCUGCGGGCCGCUgaUGagc -5'
miRNA: 3'- cGCUA-CGCUGCG--------------GGCCGCUGAUGaGC- -5'
miRNA: 3'- -CGCuaCGCUGCgGGCCGCUG---AUGaGC- -5'
miRNA: 3'- -CGCu-----ACG-----CUGCGGGCCGCUGAUGa---GC- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17080 | 3' | -57.5 | NC_004333.2 | + | 44811 | 0.7 | 0.381692 |
|
Target: 5'- aCGccGCGACGCUCGGCGAggACa-- -3' miRNA: 3'- cGCuaCGCUGCGGGCCGCUgaUGagc -5' |
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| 17080 | 3' | -57.5 | NC_004333.2 | + | 20464 | 0.69 | 0.405764 |
|
Target: 5'- cCGGUcGUGACGCagccgguuagcgucCCGGCGGCUGC-CGc -3' miRNA: 3'- cGCUA-CGCUGCG--------------GGCCGCUGAUGaGC- -5' |
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| 17080 | 3' | -57.5 | NC_004333.2 | + | 25942 | 0.69 | 0.41769 |
|
Target: 5'- cGCGgcGCGAUG-CCGGCGGCcagUACgCGg -3' miRNA: 3'- -CGCuaCGCUGCgGGCCGCUG---AUGaGC- -5' |
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| 17080 | 3' | -57.5 | NC_004333.2 | + | 20920 | 0.76 | 0.145588 |
|
Target: 5'- cGCGcaggauUGCaacccGACGCCCGGCGGCUACgccaCGg -3' miRNA: 3'- -CGCu-----ACG-----CUGCGGGCCGCUGAUGa---GC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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