Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17086 | 3' | -56.3 | NC_004333.2 | + | 13176 | 0.72 | 0.304752 |
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Target: 5'- cGAUGCCGUacaucgacaacuggcUGCGCAcacgccaGUCGGUGUCGg -3' miRNA: 3'- -CUACGGCA---------------GCGCGUa------CAGUCGCAGCa -5' |
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| 17086 | 3' | -56.3 | NC_004333.2 | + | 23838 | 0.74 | 0.227279 |
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Target: 5'- cGUGCCGuUCGCGCAgaacgacucGUCGcGCGUCGa -3' miRNA: 3'- cUACGGC-AGCGCGUa--------CAGU-CGCAGCa -5' |
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| 17086 | 3' | -56.3 | NC_004333.2 | + | 31294 | 0.74 | 0.223702 |
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Target: 5'- cGcgGCCGUCGCGCcggcAugaccaaccggcaaaUGUCGGCGUUGUc -3' miRNA: 3'- -CuaCGGCAGCGCG----U---------------ACAGUCGCAGCA- -5' |
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| 17086 | 3' | -56.3 | NC_004333.2 | + | 11884 | 0.75 | 0.183412 |
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Target: 5'- --cGCCGUCGCGCugAUGgccGCGUCGUa -3' miRNA: 3'- cuaCGGCAGCGCG--UACaguCGCAGCA- -5' |
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| 17086 | 3' | -56.3 | NC_004333.2 | + | 7343 | 0.78 | 0.111138 |
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Target: 5'- --cGCCGUCGCGCAgagaUCGGCGUCu- -3' miRNA: 3'- cuaCGGCAGCGCGUac--AGUCGCAGca -5' |
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| 17086 | 3' | -56.3 | NC_004333.2 | + | 39191 | 1.07 | 0.000922 |
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Target: 5'- cGAUGCCGUCGCGCAUGUCAGCGUCGUu -3' miRNA: 3'- -CUACGGCAGCGCGUACAGUCGCAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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