miRNA: 3'- -UAGcCCGCAUGUa-GCugU---UUGGCGCG- -5'
miRNA: 3'- uagCCCG-Ca----UGUAGC----------UGUUUGGCGCG- -5'
miRNA: 3'- uaGC-CCGCAuguAGCuGUUUGGCGCG- -5'
miRNA: 3'- uaGCCCGCauguaGCUGU-UUGGCGCG- -5'
miRNA: 3'- uaGcCCGCAUGUAGCugU-----UUGGCGCG- -5'
Results 121 - 125 of 125 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17089 | 5' | -53.6 | NC_004333.2 | + | 25867 | 0.69 | 0.585976 |
|
Target: 5'- uAUC-GGCGUGCGcgCGugAuaaGAUCGCGCg -3' miRNA: 3'- -UAGcCCGCAUGUa-GCugU---UUGGCGCG- -5' |
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| 17089 | 5' | -53.6 | NC_004333.2 | + | 32935 | 0.69 | 0.585976 |
|
Target: 5'- --aGGGCuGgcgcaACAUCGaccgacucgaGCAGGCCGCGCu -3' miRNA: 3'- uagCCCG-Ca----UGUAGC----------UGUUUGGCGCG- -5' |
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| 17089 | 5' | -53.6 | NC_004333.2 | + | 9785 | 0.69 | 0.574857 |
|
Target: 5'- -aCGcGGCGUu--UCG-CGAACCGCGUg -3' miRNA: 3'- uaGC-CCGCAuguAGCuGUUUGGCGCG- -5' |
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| 17089 | 5' | -53.6 | NC_004333.2 | + | 766 | 0.69 | 0.574857 |
|
Target: 5'- -gCGGGCGg----CGGCAcGAUCGCGCu -3' miRNA: 3'- uaGCCCGCauguaGCUGU-UUGGCGCG- -5' |
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| 17089 | 5' | -53.6 | NC_004333.2 | + | 45768 | 0.72 | 0.408403 |
|
Target: 5'- -gCaGGCGUGCGUUGugGuagcuGAUCGCGCg -3' miRNA: 3'- uaGcCCGCAUGUAGCugU-----UUGGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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