Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17091 | 5' | -62 | NC_004333.2 | + | 43501 | 0.67 | 0.302203 |
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Target: 5'- gCGCGUCCGUCagcccgacccgaGCGUcugccuGCgCGGGCG-GCUg -3' miRNA: 3'- -GUGCAGGUAG------------CGCG------CG-GCCCGCuCGA- -5' |
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| 17091 | 5' | -62 | NC_004333.2 | + | 33446 | 0.67 | 0.307311 |
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Target: 5'- --aGUCCGgcagcauuggcagcgGCGCGCCGGGCGAu-- -3' miRNA: 3'- gugCAGGUag-------------CGCGCGGCCCGCUcga -5' |
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| 17091 | 5' | -62 | NC_004333.2 | + | 5010 | 0.67 | 0.30952 |
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Target: 5'- aCACGgugccuUCCGUCGCGCuugacaggcgGCCaGGCGgccGGCUg -3' miRNA: 3'- -GUGC------AGGUAGCGCG----------CGGcCCGC---UCGA- -5' |
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| 17091 | 5' | -62 | NC_004333.2 | + | 47457 | 0.67 | 0.316971 |
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Target: 5'- gCGCGUCgGUCG-GCGCuuucguuucauCGGGCG-GCg -3' miRNA: 3'- -GUGCAGgUAGCgCGCG-----------GCCCGCuCGa -5' |
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| 17091 | 5' | -62 | NC_004333.2 | + | 21222 | 0.66 | 0.34013 |
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Target: 5'- gGCGgCCGUCGCGCGCaucuGGaaCGGGCc -3' miRNA: 3'- gUGCaGGUAGCGCGCGg---CCc-GCUCGa -5' |
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| 17091 | 5' | -62 | NC_004333.2 | + | 18796 | 0.66 | 0.348117 |
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Target: 5'- cCGCGcUUAUCGCGCgGCCgcaGGGCGcguGCUg -3' miRNA: 3'- -GUGCaGGUAGCGCG-CGG---CCCGCu--CGA- -5' |
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| 17091 | 5' | -62 | NC_004333.2 | + | 21018 | 0.66 | 0.35542 |
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Target: 5'- uGCGUCCGgcccCGUGgcguagcCGCCGGGCGucgGGUUg -3' miRNA: 3'- gUGCAGGUa---GCGC-------GCGGCCCGC---UCGA- -5' |
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| 17091 | 5' | -62 | NC_004333.2 | + | 29978 | 0.66 | 0.356238 |
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Target: 5'- cCGCGcugCCAcCG-GCGCCGaGcGCGAGCa -3' miRNA: 3'- -GUGCa--GGUaGCgCGCGGC-C-CGCUCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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