Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17091 | 5' | -62 | NC_004333.2 | + | 37429 | 0.7 | 0.202842 |
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Target: 5'- aCGCGgugCaCGUCGCGUaGCCaGGGCGAcGCa -3' miRNA: 3'- -GUGCa--G-GUAGCGCG-CGG-CCCGCU-CGa -5' |
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| 17091 | 5' | -62 | NC_004333.2 | + | 40101 | 0.71 | 0.164684 |
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Target: 5'- gGCGUCCG-CGCcgugGCGCCGcGCGAGUUc -3' miRNA: 3'- gUGCAGGUaGCG----CGCGGCcCGCUCGA- -5' |
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| 17091 | 5' | -62 | NC_004333.2 | + | 12488 | 0.71 | 0.152522 |
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Target: 5'- cUACGUCCAgcgcuuggccgaaggCggcgacacugGCGCGCCGGGCgGGGCg -3' miRNA: 3'- -GUGCAGGUa--------------G----------CGCGCGGCCCG-CUCGa -5' |
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| 17091 | 5' | -62 | NC_004333.2 | + | 41886 | 0.72 | 0.140428 |
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Target: 5'- gGCG-CCGUCGUGcCGUCGGGC-AGCUu -3' miRNA: 3'- gUGCaGGUAGCGC-GCGGCCCGcUCGA- -5' |
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| 17091 | 5' | -62 | NC_004333.2 | + | 3290 | 0.72 | 0.140428 |
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Target: 5'- gGCGU-CAUCggggcgggcgGCGCGCCGGGCcGGCUc -3' miRNA: 3'- gUGCAgGUAG----------CGCGCGGCCCGcUCGA- -5' |
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| 17091 | 5' | -62 | NC_004333.2 | + | 18568 | 0.78 | 0.049257 |
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Target: 5'- aGCGUCCGUCgGCGUGCCGgcaacGGCGGGUa -3' miRNA: 3'- gUGCAGGUAG-CGCGCGGC-----CCGCUCGa -5' |
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| 17091 | 5' | -62 | NC_004333.2 | + | 41387 | 1.06 | 0.000342 |
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Target: 5'- uCACGUCCAUCGCGCGCCGGGCGAGCUc -3' miRNA: 3'- -GUGCAGGUAGCGCGCGGCCCGCUCGA- -5' |
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| 17091 | 5' | -62 | NC_004333.2 | + | 21018 | 0.66 | 0.35542 |
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Target: 5'- uGCGUCCGgcccCGUGgcguagcCGCCGGGCGucgGGUUg -3' miRNA: 3'- gUGCAGGUa---GCGC-------GCGGCCCGC---UCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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