Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17096 | 5' | -55.7 | NC_004333.2 | + | 35987 | 0.66 | 0.636145 |
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Target: 5'- ---cUCGCGACCU-GGUucGCGGUCGc -3' miRNA: 3'- uagaAGCGCUGGAgCCAu-UGCCGGUu -5' |
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| 17096 | 5' | -55.7 | NC_004333.2 | + | 29793 | 0.66 | 0.602427 |
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Target: 5'- gGUCgUUCGCGACgaCGGUcACcgGGCCGu -3' miRNA: 3'- -UAG-AAGCGCUGgaGCCAuUG--CCGGUu -5' |
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| 17096 | 5' | -55.7 | NC_004333.2 | + | 22290 | 0.67 | 0.568947 |
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Target: 5'- cUCUUUGCGGCCcgucUCGGcaucgAGCaGGCCGc -3' miRNA: 3'- uAGAAGCGCUGG----AGCCa----UUG-CCGGUu -5' |
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| 17096 | 5' | -55.7 | NC_004333.2 | + | 11199 | 0.67 | 0.543593 |
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Target: 5'- uUC-UCGCGACCcgcgcgcacgugauUCGGgcGCGGCUc- -3' miRNA: 3'- uAGaAGCGCUGG--------------AGCCauUGCCGGuu -5' |
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| 17096 | 5' | -55.7 | NC_004333.2 | + | 32231 | 0.67 | 0.534859 |
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Target: 5'- cGUCUugagcUCGCGGauaUCGGUuggcagcAGCGGCCGAa -3' miRNA: 3'- -UAGA-----AGCGCUgg-AGCCA-------UUGCCGGUU- -5' |
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| 17096 | 5' | -55.7 | NC_004333.2 | + | 20052 | 0.68 | 0.514322 |
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Target: 5'- cUCUcUCGCaACCUCGauuGCGGCCGAc -3' miRNA: 3'- uAGA-AGCGcUGGAGCcauUGCCGGUU- -5' |
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| 17096 | 5' | -55.7 | NC_004333.2 | + | 15083 | 0.7 | 0.403044 |
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Target: 5'- -gUUUCGCGGCgUCGGcgucGGCGGCCc- -3' miRNA: 3'- uaGAAGCGCUGgAGCCa---UUGCCGGuu -5' |
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| 17096 | 5' | -55.7 | NC_004333.2 | + | 13957 | 0.71 | 0.34912 |
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Target: 5'- -cCUUgGCGACUUCaGGcGGCGGCCGGu -3' miRNA: 3'- uaGAAgCGCUGGAG-CCaUUGCCGGUU- -5' |
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| 17096 | 5' | -55.7 | NC_004333.2 | + | 44536 | 0.74 | 0.224744 |
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Target: 5'- -aCUUCGCGcCCUCGGUGuccuuuugcaccgGCaGGCCGAg -3' miRNA: 3'- uaGAAGCGCuGGAGCCAU-------------UG-CCGGUU- -5' |
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| 17096 | 5' | -55.7 | NC_004333.2 | + | 44850 | 1.04 | 0.001367 |
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Target: 5'- gAUCUUCGCGACCUCGGUAACGGCCAAg -3' miRNA: 3'- -UAGAAGCGCUGGAGCCAUUGCCGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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