Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17101 | 3' | -55.8 | NC_004333.2 | + | 30744 | 0.66 | 0.603201 |
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Target: 5'- ---gCGGCGGCGCGaaCGGCUucAUGAAg -3' miRNA: 3'- uagaGCCGCUGCGC--GUCGAcuUACUU- -5' |
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| 17101 | 3' | -55.8 | NC_004333.2 | + | 47975 | 0.66 | 0.591911 |
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Target: 5'- cGUCaUCGGCGGUGUGCGGCUGu----- -3' miRNA: 3'- -UAG-AGCCGCUGCGCGUCGACuuacuu -5' |
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| 17101 | 3' | -55.8 | NC_004333.2 | + | 47393 | 0.66 | 0.562747 |
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Target: 5'- gAUCUCGGCuuuCGUGCGGCgcuugcgaggaggcuUGAcgGAAg -3' miRNA: 3'- -UAGAGCCGcu-GCGCGUCG---------------ACUuaCUU- -5' |
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| 17101 | 3' | -55.8 | NC_004333.2 | + | 45041 | 0.67 | 0.525234 |
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Target: 5'- ---cUGGCGGCGCGCAaacGCUGAAc--- -3' miRNA: 3'- uagaGCCGCUGCGCGU---CGACUUacuu -5' |
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| 17101 | 3' | -55.8 | NC_004333.2 | + | 4290 | 0.68 | 0.441296 |
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Target: 5'- uUCUCGaGCGucaACGCGCAGCUGcAAa--- -3' miRNA: 3'- uAGAGC-CGC---UGCGCGUCGAC-UUacuu -5' |
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| 17101 | 3' | -55.8 | NC_004333.2 | + | 3312 | 0.75 | 0.179564 |
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Target: 5'- ---gCGGCGGCGCGCGGUUGAAUu-- -3' miRNA: 3'- uagaGCCGCUGCGCGUCGACUUAcuu -5' |
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| 17101 | 3' | -55.8 | NC_004333.2 | + | 15350 | 0.75 | 0.179564 |
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Target: 5'- gAUCUCGGCgcaGACGCGCGcGCUGAAc--- -3' miRNA: 3'- -UAGAGCCG---CUGCGCGU-CGACUUacuu -5' |
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| 17101 | 3' | -55.8 | NC_004333.2 | + | 28207 | 0.75 | 0.16051 |
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Target: 5'- -cCUCGGCGGCGCGCAGU--AcgGAAa -3' miRNA: 3'- uaGAGCCGCUGCGCGUCGacUuaCUU- -5' |
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| 17101 | 3' | -55.8 | NC_004333.2 | + | 47675 | 1.04 | 0.001341 |
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Target: 5'- cAUCUCGGCGACGCGCAGCUGAAUGAAc -3' miRNA: 3'- -UAGAGCCGCUGCGCGUCGACUUACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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