Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17103 | 3' | -57 | NC_004333.2 | + | 14335 | 0.69 | 0.399132 |
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Target: 5'- cUCGAAGgcGUGCCG-CCGCuGGCu- -3' miRNA: 3'- aGGUUUCuuCGCGGCaGGCGuCCGcc -5' |
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| 17103 | 3' | -57 | NC_004333.2 | + | 11645 | 0.7 | 0.389206 |
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Target: 5'- gUCGAAGuAGCGCUgccaguaGUCgCGCAGGCGc -3' miRNA: 3'- aGGUUUCuUCGCGG-------CAG-GCGUCCGCc -5' |
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| 17103 | 3' | -57 | NC_004333.2 | + | 12472 | 0.7 | 0.351988 |
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Target: 5'- gCCGAAGGcggcgacacuggcGCGCCGggcggggCGCGGGCGGg -3' miRNA: 3'- aGGUUUCUu------------CGCGGCag-----GCGUCCGCC- -5' |
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| 17103 | 3' | -57 | NC_004333.2 | + | 9239 | 0.72 | 0.264587 |
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Target: 5'- gCCGAacGGggGCGCCucgCCGCGuGCGGc -3' miRNA: 3'- aGGUU--UCuuCGCGGca-GGCGUcCGCC- -5' |
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| 17103 | 3' | -57 | NC_004333.2 | + | 27323 | 0.72 | 0.264587 |
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Target: 5'- gUCCGAAuacGAGGCGUCGg-CGCAGGCa- -3' miRNA: 3'- -AGGUUU---CUUCGCGGCagGCGUCCGcc -5' |
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| 17103 | 3' | -57 | NC_004333.2 | + | 3942 | 0.73 | 0.245051 |
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Target: 5'- aCCGGcAGGuuGGCGCCGuUCUGCAGGUaGGa -3' miRNA: 3'- aGGUU-UCU--UCGCGGC-AGGCGUCCG-CC- -5' |
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| 17103 | 3' | -57 | NC_004333.2 | + | 30769 | 0.73 | 0.238811 |
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Target: 5'- uUCCAGGacGAAGuUGCCG-CCGCcGGCGGc -3' miRNA: 3'- -AGGUUU--CUUC-GCGGCaGGCGuCCGCC- -5' |
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| 17103 | 3' | -57 | NC_004333.2 | + | 26149 | 0.75 | 0.173876 |
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Target: 5'- cCCGAauccGGAAGCGCCG-CCGCugcGGCGc -3' miRNA: 3'- aGGUU----UCUUCGCGGCaGGCGu--CCGCc -5' |
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| 17103 | 3' | -57 | NC_004333.2 | + | 42839 | 0.75 | 0.164718 |
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Target: 5'- gUCCGAAGAAgcccGCGCCGcaUCUGCcagugagacggGGGCGGa -3' miRNA: 3'- -AGGUUUCUU----CGCGGC--AGGCG-----------UCCGCC- -5' |
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| 17103 | 3' | -57 | NC_004333.2 | + | 47897 | 1.1 | 0.000547 |
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Target: 5'- cUCCAAAGAAGCGCCGUCCGCAGGCGGc -3' miRNA: 3'- -AGGUUUCUUCGCGGCAGGCGUCCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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