Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17106 | 3' | -55 | NC_004333.2 | + | 20458 | 0.71 | 0.394991 |
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Target: 5'- gCCACUGCGGCCa--CGGCCUUa--- -3' miRNA: 3'- aGGUGACGUCGGgaaGCUGGAAcucg -5' |
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| 17106 | 3' | -55 | NC_004333.2 | + | 40531 | 0.71 | 0.377658 |
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Target: 5'- cCCGCUGCAGCCCcguacuugacuuucUUUGAUCUgucccgcuuaguagGAGUg -3' miRNA: 3'- aGGUGACGUCGGG--------------AAGCUGGAa-------------CUCG- -5' |
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| 17106 | 3' | -55 | NC_004333.2 | + | 31906 | 0.72 | 0.333725 |
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Target: 5'- cCCGCUGCAGCUCUacacgcugaucUCG-CCgcaGGGCa -3' miRNA: 3'- aGGUGACGUCGGGA-----------AGCuGGaa-CUCG- -5' |
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| 17106 | 3' | -55 | NC_004333.2 | + | 2944 | 0.73 | 0.309684 |
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Target: 5'- gCCGCUGUAGCCCUgCaGCUgcgcGAGCg -3' miRNA: 3'- aGGUGACGUCGGGAaGcUGGaa--CUCG- -5' |
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| 17106 | 3' | -55 | NC_004333.2 | + | 39831 | 0.76 | 0.187638 |
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Target: 5'- gUCCACUGCAgGCCCUUgaugcggcaGACCaucGAGCa -3' miRNA: 3'- -AGGUGACGU-CGGGAAg--------CUGGaa-CUCG- -5' |
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| 17106 | 3' | -55 | NC_004333.2 | + | 775 | 1.13 | 0.00043 |
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Target: 5'- gUCCACUGCAGCCCUUCGACCUUGAGCg -3' miRNA: 3'- -AGGUGACGUCGGGAAGCUGGAACUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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