Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17109 | 3' | -57.6 | NC_004333.2 | + | 8551 | 0.67 | 0.499038 |
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Target: 5'- gUGGCuGGcUCGCCG-UCGcGAGGUACg -3' miRNA: 3'- -ACCGcCCcAGCGGCuAGUcCUUCAUG- -5' |
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| 17109 | 3' | -57.6 | NC_004333.2 | + | 14271 | 0.68 | 0.448723 |
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Target: 5'- cGGCGGca-CGCCuucgagcAUCGGGAAGUGCu -3' miRNA: 3'- aCCGCCccaGCGGc------UAGUCCUUCAUG- -5' |
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| 17109 | 3' | -57.6 | NC_004333.2 | + | 41766 | 0.68 | 0.429393 |
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Target: 5'- gGGCuauGGGUCuGCaCGAgcgAGGAAGUGCg -3' miRNA: 3'- aCCGc--CCCAG-CG-GCUag-UCCUUCAUG- -5' |
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| 17109 | 3' | -57.6 | NC_004333.2 | + | 2015 | 0.71 | 0.27273 |
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Target: 5'- aGGCGGcccgcGUCGCCGGUgCGGGccagcuGGUGCa -3' miRNA: 3'- aCCGCCc----CAGCGGCUA-GUCCu-----UCAUG- -5' |
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| 17109 | 3' | -57.6 | NC_004333.2 | + | 22984 | 0.71 | 0.265897 |
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Target: 5'- -cGCGGuaccGGUCGCCGAgagCAGGAAGc-- -3' miRNA: 3'- acCGCC----CCAGCGGCUa--GUCCUUCaug -5' |
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| 17109 | 3' | -57.6 | NC_004333.2 | + | 1893 | 1.08 | 0.00054 |
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Target: 5'- uUGGCGGGGUCGCCGAUCAGGAAGUACc -3' miRNA: 3'- -ACCGCCCCAGCGGCUAGUCCUUCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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