Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17118 | 5' | -55.5 | NC_004333.2 | + | 3430 | 0.66 | 0.593961 |
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Target: 5'- uGCGGCGCGGUcucCaCCUGGUUGAc -3' miRNA: 3'- uCGUCGCGUCGaacGcGGACUAACUu -5' |
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| 17118 | 5' | -55.5 | NC_004333.2 | + | 30633 | 0.66 | 0.559923 |
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Target: 5'- cAGCAGCGCGGCaagGCGCg-GcgUGc- -3' miRNA: 3'- -UCGUCGCGUCGaa-CGCGgaCuaACuu -5' |
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| 17118 | 5' | -55.5 | NC_004333.2 | + | 33410 | 0.66 | 0.54869 |
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Target: 5'- uGCGGCGCAGCgacugcGCGCC-GAa---- -3' miRNA: 3'- uCGUCGCGUCGaa----CGCGGaCUaacuu -5' |
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| 17118 | 5' | -55.5 | NC_004333.2 | + | 29107 | 0.67 | 0.526448 |
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Target: 5'- gGGCGGCGCGGUgcuCGCgCUGGUUa-- -3' miRNA: 3'- -UCGUCGCGUCGaacGCG-GACUAAcuu -5' |
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| 17118 | 5' | -55.5 | NC_004333.2 | + | 20445 | 0.7 | 0.355901 |
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Target: 5'- cGGCGGCGCgaAGCcUGCGCCgGucgUGAc -3' miRNA: 3'- -UCGUCGCG--UCGaACGCGGaCua-ACUu -5' |
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| 17118 | 5' | -55.5 | NC_004333.2 | + | 4189 | 0.71 | 0.297865 |
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Target: 5'- cAGCGGCGUGuGCUucUGCGCCUGGa---- -3' miRNA: 3'- -UCGUCGCGU-CGA--ACGCGGACUaacuu -5' |
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| 17118 | 5' | -55.5 | NC_004333.2 | + | 36097 | 0.73 | 0.210031 |
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Target: 5'- gAGUAGUGCAGCUgugaGCCUGAUcgUGAu -3' miRNA: 3'- -UCGUCGCGUCGAacg-CGGACUA--ACUu -5' |
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| 17118 | 5' | -55.5 | NC_004333.2 | + | 9535 | 0.74 | 0.182678 |
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Target: 5'- cGGCGGUGCGGCacccGCGCCUGGUg--- -3' miRNA: 3'- -UCGUCGCGUCGaa--CGCGGACUAacuu -5' |
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| 17118 | 5' | -55.5 | NC_004333.2 | + | 5167 | 1.06 | 0.00086 |
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Target: 5'- gAGCAGCGCAGCUUGCGCCUGAUUGAAc -3' miRNA: 3'- -UCGUCGCGUCGAACGCGGACUAACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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