Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17125 | 3' | -52.1 | NC_004333.2 | + | 16615 | 0.66 | 0.840641 |
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Target: 5'- aCGGACGccgGGcAGUUgcGCcacCGCUCGCg -3' miRNA: 3'- -GCCUGC---UCaUCAAauCGa--GCGAGCGg -5' |
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| 17125 | 3' | -52.1 | NC_004333.2 | + | 30381 | 0.67 | 0.79413 |
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Target: 5'- -uGGCGGGUGGUgucgcaggcgcgacGGCggccaGCUCGCCg -3' miRNA: 3'- gcCUGCUCAUCAaa------------UCGag---CGAGCGG- -5' |
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| 17125 | 3' | -52.1 | NC_004333.2 | + | 12448 | 0.68 | 0.772817 |
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Target: 5'- cCGGGCGGGgcGcg-GGCgggCGCUacggCGCCc -3' miRNA: 3'- -GCCUGCUCauCaaaUCGa--GCGA----GCGG- -5' |
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| 17125 | 3' | -52.1 | NC_004333.2 | + | 36310 | 0.68 | 0.762425 |
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Target: 5'- cCGccCGAGUAGUcgccgGGCgUCGCaUCGCCu -3' miRNA: 3'- -GCcuGCUCAUCAaa---UCG-AGCG-AGCGG- -5' |
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| 17125 | 3' | -52.1 | NC_004333.2 | + | 25711 | 0.69 | 0.719577 |
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Target: 5'- --cGCGAGaa---UGGCUUGCUCGCCg -3' miRNA: 3'- gccUGCUCaucaaAUCGAGCGAGCGG- -5' |
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| 17125 | 3' | -52.1 | NC_004333.2 | + | 20983 | 0.69 | 0.71739 |
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Target: 5'- gCGGGCGAGUGGgugcguuucgAGCgCGUggcaGCCg -3' miRNA: 3'- -GCCUGCUCAUCaaa-------UCGaGCGag--CGG- -5' |
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| 17125 | 3' | -52.1 | NC_004333.2 | + | 7954 | 0.71 | 0.607748 |
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Target: 5'- uCGGACGAGUAGUcgccauacgUGGCaacCUCGUCg -3' miRNA: 3'- -GCCUGCUCAUCAa--------AUCGagcGAGCGG- -5' |
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| 17125 | 3' | -52.1 | NC_004333.2 | + | 45526 | 0.73 | 0.456302 |
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Target: 5'- uGGACGccGUAGUUggccgccauGCUCGCgaaugCGCCg -3' miRNA: 3'- gCCUGCu-CAUCAAau-------CGAGCGa----GCGG- -5' |
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| 17125 | 3' | -52.1 | NC_004333.2 | + | 8560 | 0.74 | 0.436244 |
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Target: 5'- gCGGACGuug----UGGCUgGCUCGCCg -3' miRNA: 3'- -GCCUGCucaucaaAUCGAgCGAGCGG- -5' |
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| 17125 | 3' | -52.1 | NC_004333.2 | + | 7813 | 1.14 | 0.000977 |
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Target: 5'- gCGGACGAGUAGUUUAGCUCGCUCGCCa -3' miRNA: 3'- -GCCUGCUCAUCAAAUCGAGCGAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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