Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17128 | 3' | -53.8 | NC_004333.2 | + | 8936 | 0.68 | 0.677733 |
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Target: 5'- -cUUCGUUCGCGcCGGCGcUGUCc-- -3' miRNA: 3'- gcAAGCAAGCGCuGCCGUcACAGcua -5' |
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| 17128 | 3' | -53.8 | NC_004333.2 | + | 29790 | 0.68 | 0.677733 |
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Target: 5'- gCGggUCGUUCGCGACGaCGGUcaccgggccGUUGAUg -3' miRNA: 3'- -GCa-AGCAAGCGCUGCcGUCA---------CAGCUA- -5' |
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| 17128 | 3' | -53.8 | NC_004333.2 | + | 32100 | 0.68 | 0.666633 |
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Target: 5'- uCGgcgCGUUCaGUGACGGCAGUucCGAc -3' miRNA: 3'- -GCaa-GCAAG-CGCUGCCGUCAcaGCUa -5' |
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| 17128 | 3' | -53.8 | NC_004333.2 | + | 29855 | 0.68 | 0.666633 |
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Target: 5'- uCGUUCGcagcgaagCGCGcGCGGUAGUcGUCGGc -3' miRNA: 3'- -GCAAGCaa------GCGC-UGCCGUCA-CAGCUa -5' |
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| 17128 | 3' | -53.8 | NC_004333.2 | + | 3672 | 0.68 | 0.644344 |
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Target: 5'- --cUCGUUCGCGACGGUcugAGccGUCGc- -3' miRNA: 3'- gcaAGCAAGCGCUGCCG---UCa-CAGCua -5' |
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| 17128 | 3' | -53.8 | NC_004333.2 | + | 39716 | 0.69 | 0.61085 |
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Target: 5'- cCGUgaaGUUCGCGucCGGCAGcaGUCGGc -3' miRNA: 3'- -GCAag-CAAGCGCu-GCCGUCa-CAGCUa -5' |
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| 17128 | 3' | -53.8 | NC_004333.2 | + | 25247 | 0.76 | 0.231167 |
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Target: 5'- aGUUCGagaCGCGGCGGCAggcgagcGUGUCGAUc -3' miRNA: 3'- gCAAGCaa-GCGCUGCCGU-------CACAGCUA- -5' |
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| 17128 | 3' | -53.8 | NC_004333.2 | + | 9182 | 1.05 | 0.00231 |
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Target: 5'- gCGUUCGUUCGCGACGGCAGUGUCGAUc -3' miRNA: 3'- -GCAAGCAAGCGCUGCCGUCACAGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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