Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17140 | 5' | -51.5 | NC_004333.2 | + | 18109 | 0.66 | 0.847959 |
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Target: 5'- gCGGGCAGCgcGAUCAGCGc------ -3' miRNA: 3'- gGCUCGUCGaaCUAGUCGCcuuacac -5' |
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| 17140 | 5' | -51.5 | NC_004333.2 | + | 14676 | 0.66 | 0.847959 |
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Target: 5'- uUCGcGCAGCUUG-UCGGCcGAGUGc- -3' miRNA: 3'- -GGCuCGUCGAACuAGUCGcCUUACac -5' |
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| 17140 | 5' | -51.5 | NC_004333.2 | + | 47837 | 0.67 | 0.829672 |
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Target: 5'- uUCGAGCGcggcgauguGCUUGGcacagUCGGCcGGggUGUa -3' miRNA: 3'- -GGCUCGU---------CGAACU-----AGUCG-CCuuACAc -5' |
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| 17140 | 5' | -51.5 | NC_004333.2 | + | 9549 | 0.67 | 0.829672 |
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Target: 5'- cUCGAGCAGC----UCGGCGGcg-GUGc -3' miRNA: 3'- -GGCUCGUCGaacuAGUCGCCuuaCAC- -5' |
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| 17140 | 5' | -51.5 | NC_004333.2 | + | 41872 | 0.67 | 0.810488 |
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Target: 5'- gUCGGGCAGCUUGAcCGGaaaCGGAcUGc- -3' miRNA: 3'- -GGCUCGUCGAACUaGUC---GCCUuACac -5' |
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| 17140 | 5' | -51.5 | NC_004333.2 | + | 17547 | 0.68 | 0.780227 |
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Target: 5'- gCGAGCAGCgUGAgcgCGGCGGc----- -3' miRNA: 3'- gGCUCGUCGaACUa--GUCGCCuuacac -5' |
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| 17140 | 5' | -51.5 | NC_004333.2 | + | 37727 | 0.68 | 0.769791 |
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Target: 5'- gUCGGGCGcgcGCUcgaugaaugucUGAUcCGGCGGGAUGUc -3' miRNA: 3'- -GGCUCGU---CGA-----------ACUA-GUCGCCUUACAc -5' |
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| 17140 | 5' | -51.5 | NC_004333.2 | + | 38233 | 0.69 | 0.726646 |
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Target: 5'- gCGAGCGGCgugccaaGAUCGGCGGccggGUc -3' miRNA: 3'- gGCUCGUCGaa-----CUAGUCGCCuua-CAc -5' |
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| 17140 | 5' | -51.5 | NC_004333.2 | + | 46839 | 0.7 | 0.670517 |
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Target: 5'- gCCGAGCAGCg----CGGCGGAc---- -3' miRNA: 3'- -GGCUCGUCGaacuaGUCGCCUuacac -5' |
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| 17140 | 5' | -51.5 | NC_004333.2 | + | 12822 | 1.11 | 0.001569 |
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Target: 5'- aCCGAGCAGCUUGAUCAGCGGAAUGUGc -3' miRNA: 3'- -GGCUCGUCGAACUAGUCGCCUUACAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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