Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17149 | 3' | -56.4 | NC_004333.2 | + | 41920 | 0.7 | 0.406553 |
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Target: 5'- gAUCG-GGAaacgGAUGCccGACCGCUCGCGGc -3' miRNA: 3'- -UAGCaUCUg---CUACG--CUGGCGGGUGCC- -5' |
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| 17149 | 3' | -56.4 | NC_004333.2 | + | 24923 | 0.7 | 0.397409 |
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Target: 5'- -cCGU--GCGGuucgUGCGGCCGCCgACGGu -3' miRNA: 3'- uaGCAucUGCU----ACGCUGGCGGgUGCC- -5' |
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| 17149 | 3' | -56.4 | NC_004333.2 | + | 27031 | 0.7 | 0.397409 |
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Target: 5'- gAUCGUcGAUGAUGCGcACgGCCUuuaccucgACGGg -3' miRNA: 3'- -UAGCAuCUGCUACGC-UGgCGGG--------UGCC- -5' |
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| 17149 | 3' | -56.4 | NC_004333.2 | + | 20916 | 0.7 | 0.397409 |
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Target: 5'- uUCG-AGcAgGAUGCGcgGCCGCCCGuCGGg -3' miRNA: 3'- uAGCaUC-UgCUACGC--UGGCGGGU-GCC- -5' |
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| 17149 | 3' | -56.4 | NC_004333.2 | + | 11977 | 0.7 | 0.379521 |
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Target: 5'- -gCGUcGACGAUcGCGGCCGUaUCGCGGu -3' miRNA: 3'- uaGCAuCUGCUA-CGCUGGCG-GGUGCC- -5' |
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| 17149 | 3' | -56.4 | NC_004333.2 | + | 2474 | 0.7 | 0.379521 |
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Target: 5'- -cUGUAcGGCGAccagGCGACCGCCgGCGc -3' miRNA: 3'- uaGCAU-CUGCUa---CGCUGGCGGgUGCc -5' |
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| 17149 | 3' | -56.4 | NC_004333.2 | + | 9540 | 0.73 | 0.276902 |
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Target: 5'- cUCGgcGGCGGUGCGGCa-CCCGCGc -3' miRNA: 3'- uAGCauCUGCUACGCUGgcGGGUGCc -5' |
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| 17149 | 3' | -56.4 | NC_004333.2 | + | 36393 | 0.78 | 0.124386 |
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Target: 5'- -cCGUAGGCGAUGCGA-CGCCCGgCGa -3' miRNA: 3'- uaGCAUCUGCUACGCUgGCGGGU-GCc -5' |
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| 17149 | 3' | -56.4 | NC_004333.2 | + | 15902 | 1.08 | 0.000897 |
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Target: 5'- cAUCGUAGACGAUGCGACCGCCCACGGu -3' miRNA: 3'- -UAGCAUCUGCUACGCUGGCGGGUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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