Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17153 | 3' | -60.9 | NC_004333.2 | + | 8656 | 0.69 | 0.262347 |
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Target: 5'- gCGCCCGGcuUGCuCGUGacgguGCCGuCGgCGUUCa -3' miRNA: 3'- -GCGGGCC--ACG-GCAC-----CGGCuGU-GCAAG- -5' |
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| 17153 | 3' | -60.9 | NC_004333.2 | + | 15364 | 0.69 | 0.255914 |
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Target: 5'- gCGCCCGGUGCUGauGCUGAC-CGcgCc -3' miRNA: 3'- -GCGGGCCACGGCacCGGCUGuGCaaG- -5' |
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| 17153 | 3' | -60.9 | NC_004333.2 | + | 12348 | 0.69 | 0.255914 |
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Target: 5'- gCGCcgCCGGUGCCGUGuacguuGUCGACGCa--- -3' miRNA: 3'- -GCG--GGCCACGGCAC------CGGCUGUGcaag -5' |
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| 17153 | 3' | -60.9 | NC_004333.2 | + | 38714 | 0.69 | 0.243436 |
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Target: 5'- uGCCC-GUGCCGcccgGGCCGAUcagaACGggCa -3' miRNA: 3'- gCGGGcCACGGCa---CCGGCUG----UGCaaG- -5' |
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| 17153 | 3' | -60.9 | NC_004333.2 | + | 27385 | 0.71 | 0.198516 |
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Target: 5'- uGCCCGGcgcgacgGCCGU-GCCGuCAUGUUUg -3' miRNA: 3'- gCGGGCCa------CGGCAcCGGCuGUGCAAG- -5' |
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| 17153 | 3' | -60.9 | NC_004333.2 | + | 31615 | 0.71 | 0.193439 |
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Target: 5'- cCGUCuCGGccGCUGUGGCCGcGCGCGUg- -3' miRNA: 3'- -GCGG-GCCa-CGGCACCGGC-UGUGCAag -5' |
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| 17153 | 3' | -60.9 | NC_004333.2 | + | 26561 | 0.72 | 0.176092 |
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Target: 5'- cCGCgCCGGUcuucgucccuGCgaucgugucguugaaCGUGGUCGGCGCGUUCa -3' miRNA: 3'- -GCG-GGCCA----------CG---------------GCACCGGCUGUGCAAG- -5' |
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| 17153 | 3' | -60.9 | NC_004333.2 | + | 25499 | 0.73 | 0.148652 |
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Target: 5'- aCGCCCGGcgugacgGCCGccGCCGGCACGc-- -3' miRNA: 3'- -GCGGGCCa------CGGCacCGGCUGUGCaag -5' |
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| 17153 | 3' | -60.9 | NC_004333.2 | + | 17631 | 1.09 | 0.000245 |
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Target: 5'- gCGCCCGGUGCCGUGGCCGACACGUUCg -3' miRNA: 3'- -GCGGGCCACGGCACCGGCUGUGCAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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