Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17153 | 5' | -49.7 | NC_004333.2 | + | 36309 | 0.66 | 0.942376 |
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Target: 5'- uCGGcGAGGUcuacuaUGACGCGACGCAaGGCa -3' miRNA: 3'- -GCU-UUCCAca----ACUGUGCUGUGUcCUG- -5' |
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| 17153 | 5' | -49.7 | NC_004333.2 | + | 21419 | 0.66 | 0.931463 |
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Target: 5'- cCGGAAuGGgcaaaUUGG-GCGGCGCAGGACg -3' miRNA: 3'- -GCUUU-CCac---AACUgUGCUGUGUCCUG- -5' |
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| 17153 | 5' | -49.7 | NC_004333.2 | + | 13357 | 0.67 | 0.898888 |
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Target: 5'- ----cGGUGUacgGGCGCGGCACgAGcGGCg -3' miRNA: 3'- gcuuuCCACAa--CUGUGCUGUG-UC-CUG- -5' |
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| 17153 | 5' | -49.7 | NC_004333.2 | + | 11804 | 0.68 | 0.88378 |
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Target: 5'- cCGGucGGcUGccgGACAUcGCGCAGGACg -3' miRNA: 3'- -GCUuuCC-ACaa-CUGUGcUGUGUCCUG- -5' |
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| 17153 | 5' | -49.7 | NC_004333.2 | + | 30212 | 0.7 | 0.782281 |
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Target: 5'- aCGAAAGGcGgcGGCcaagGCG-CGCAGGGCa -3' miRNA: 3'- -GCUUUCCaCaaCUG----UGCuGUGUCCUG- -5' |
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| 17153 | 5' | -49.7 | NC_004333.2 | + | 17667 | 1.12 | 0.002243 |
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Target: 5'- gCGAAAGGUGUUGACACGACACAGGACa -3' miRNA: 3'- -GCUUUCCACAACUGUGCUGUGUCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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