Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17168 | 5' | -54.5 | NC_004333.2 | + | 3294 | 0.67 | 0.672921 |
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Target: 5'- --gCCCGgcgUCAUCGGggCGGGcGGCgCGCc -3' miRNA: 3'- gaaGGGC---AGUAGCCa-GCUCuUCG-GCG- -5' |
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| 17168 | 5' | -54.5 | NC_004333.2 | + | 44224 | 0.68 | 0.595711 |
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Target: 5'- --gCgCGUCGUCGGaucgUCGAGAuagcGGCCGa -3' miRNA: 3'- gaaGgGCAGUAGCC----AGCUCU----UCGGCg -5' |
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| 17168 | 5' | -54.5 | NC_004333.2 | + | 10124 | 0.69 | 0.541286 |
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Target: 5'- -aUCCCGaCAcgcugaagUCGuUCGAGggGCUGCc -3' miRNA: 3'- gaAGGGCaGU--------AGCcAGCUCuuCGGCG- -5' |
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| 17168 | 5' | -54.5 | NC_004333.2 | + | 40307 | 0.69 | 0.541286 |
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Target: 5'- --aUUCGUCgAUCGGUUGGccGGCCGCg -3' miRNA: 3'- gaaGGGCAG-UAGCCAGCUcuUCGGCG- -5' |
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| 17168 | 5' | -54.5 | NC_004333.2 | + | 40463 | 0.7 | 0.498973 |
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Target: 5'- --gCCCGUCuucaacCGGguUCGAGucGGCCGCg -3' miRNA: 3'- gaaGGGCAGua----GCC--AGCUCu-UCGGCG- -5' |
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| 17168 | 5' | -54.5 | NC_004333.2 | + | 43064 | 0.72 | 0.382413 |
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Target: 5'- -----aGcCAUCGGUCGAGAGcGCCGCc -3' miRNA: 3'- gaagggCaGUAGCCAGCUCUU-CGGCG- -5' |
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| 17168 | 5' | -54.5 | NC_004333.2 | + | 42290 | 0.76 | 0.226219 |
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Target: 5'- ---gCCGUCGUgCGGUCGAagucGAGCCGCa -3' miRNA: 3'- gaagGGCAGUA-GCCAGCUc---UUCGGCG- -5' |
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| 17168 | 5' | -54.5 | NC_004333.2 | + | 22281 | 0.79 | 0.150778 |
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Target: 5'- --gCCCGUC-UCGGcaUCGAGcAGGCCGCa -3' miRNA: 3'- gaaGGGCAGuAGCC--AGCUC-UUCGGCG- -5' |
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| 17168 | 5' | -54.5 | NC_004333.2 | + | 9058 | 0.66 | 0.7377 |
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Target: 5'- ---gCCG-CGUCGGUCGcc--GCCGCg -3' miRNA: 3'- gaagGGCaGUAGCCAGCucuuCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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