Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17168 | 5' | -54.5 | NC_004333.2 | + | 2740 | 0.68 | 0.628805 |
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Target: 5'- --gCCCGUCA-CaGcCGAGAucgcGGCCGCc -3' miRNA: 3'- gaaGGGCAGUaGcCaGCUCU----UCGGCG- -5' |
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| 17168 | 5' | -54.5 | NC_004333.2 | + | 36354 | 0.69 | 0.540212 |
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Target: 5'- aUUgCCGUaagCAUCGGUCGuGAuaucccaGGUCGCg -3' miRNA: 3'- gAAgGGCA---GUAGCCAGCuCU-------UCGGCG- -5' |
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| 17168 | 5' | -54.5 | NC_004333.2 | + | 15853 | 0.7 | 0.498973 |
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Target: 5'- -gUCCCGUCAUCuuccuuGUgCGAGcccgcgagcGAGCCGCc -3' miRNA: 3'- gaAGGGCAGUAGc-----CA-GCUC---------UUCGGCG- -5' |
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| 17168 | 5' | -54.5 | NC_004333.2 | + | 14981 | 0.71 | 0.458196 |
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Target: 5'- ---aCCGaCGUCGaUCGAGAAGCCGg -3' miRNA: 3'- gaagGGCaGUAGCcAGCUCUUCGGCg -5' |
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| 17168 | 5' | -54.5 | NC_004333.2 | + | 41224 | 0.71 | 0.438485 |
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Target: 5'- --aCCUGUCGcCGGUCGuGucGCCGUc -3' miRNA: 3'- gaaGGGCAGUaGCCAGCuCuuCGGCG- -5' |
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| 17168 | 5' | -54.5 | NC_004333.2 | + | 23167 | 0.73 | 0.373543 |
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Target: 5'- --gCCCGUCAcgUCGGUCGGugcGCCGUu -3' miRNA: 3'- gaaGGGCAGU--AGCCAGCUcuuCGGCG- -5' |
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| 17168 | 5' | -54.5 | NC_004333.2 | + | 31106 | 0.73 | 0.364812 |
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Target: 5'- --gCCCGUCAgCGGcgCGAGuuGCUGCu -3' miRNA: 3'- gaaGGGCAGUaGCCa-GCUCuuCGGCG- -5' |
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| 17168 | 5' | -54.5 | NC_004333.2 | + | 24727 | 1.11 | 0.000802 |
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Target: 5'- uCUUCCCGUCAUCGGUCGAGAAGCCGCu -3' miRNA: 3'- -GAAGGGCAGUAGCCAGCUCUUCGGCG- -5' |
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| 17168 | 5' | -54.5 | NC_004333.2 | + | 18777 | 0.66 | 0.762671 |
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Target: 5'- cCUUCCCGcCAUCGcugaaccgcgcuuaUCGcGcGGCCGCa -3' miRNA: 3'- -GAAGGGCaGUAGCc-------------AGCuCuUCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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