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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17173 | 5' | -55 | NC_004333.2 | + | 22578 | 0.66 | 0.661785 |
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Target: 5'- aUCGCCaUCgGCUGGuAagGAGCGCAGa -3' miRNA: 3'- cAGUGGaAGaCGGCC-UagCUCGUGUU- -5' |
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| 17173 | 5' | -55 | NC_004333.2 | + | 34876 | 0.66 | 0.639155 |
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Target: 5'- -gCACCUUCgaUGCUaugagucuGGGUCGAGCAgGu -3' miRNA: 3'- caGUGGAAG--ACGG--------CCUAGCUCGUgUu -5' |
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| 17173 | 5' | -55 | NC_004333.2 | + | 20160 | 0.66 | 0.627824 |
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Target: 5'- --aGCCUacUCggccGCCGGcUCGGGCGCAc -3' miRNA: 3'- cagUGGA--AGa---CGGCCuAGCUCGUGUu -5' |
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| 17173 | 5' | -55 | NC_004333.2 | + | 4443 | 0.67 | 0.560276 |
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Target: 5'- cGUCGCa-UCUGCUGaauucGAUCGAGCAgGAg -3' miRNA: 3'- -CAGUGgaAGACGGC-----CUAGCUCGUgUU- -5' |
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| 17173 | 5' | -55 | NC_004333.2 | + | 48188 | 0.69 | 0.484313 |
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Target: 5'- gGUCACCa--UGCCGGcgCGGuGCGCGc -3' miRNA: 3'- -CAGUGGaagACGGCCuaGCU-CGUGUu -5' |
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| 17173 | 5' | -55 | NC_004333.2 | + | 11859 | 0.69 | 0.484313 |
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Target: 5'- aUCGCUUccgUCUGCCGGucggccgaaaCGGGCGCGAg -3' miRNA: 3'- cAGUGGA---AGACGGCCua--------GCUCGUGUU- -5' |
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| 17173 | 5' | -55 | NC_004333.2 | + | 26885 | 1.05 | 0.001314 |
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Target: 5'- uGUCACCUUCUGCCGGAUCGAGCACAAc -3' miRNA: 3'- -CAGUGGAAGACGGCCUAGCUCGUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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