Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17178 | 3' | -54.9 | NC_004333.2 | + | 8098 | 0.69 | 0.589882 |
Target: 5'- -aCGGGUCGacguaGGUCGuCAGGuagUUCGGg -3' miRNA: 3'- cgGCCUAGCg----CCAGC-GUCUua-AGGCC- -5' |
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17178 | 3' | -54.9 | NC_004333.2 | + | 2884 | 0.7 | 0.504688 |
Target: 5'- cGCCGGAuuUCGUGGUCGCGuacGAug-CCc- -3' miRNA: 3'- -CGGCCU--AGCGCCAGCGU---CUuaaGGcc -5' |
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17178 | 3' | -54.9 | NC_004333.2 | + | 10087 | 0.73 | 0.329181 |
Target: 5'- gGUCGGAUCGCGGuacaggUCGUAGAcgcgaUUCGGa -3' miRNA: 3'- -CGGCCUAGCGCC------AGCGUCUua---AGGCC- -5' |
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17178 | 3' | -54.9 | NC_004333.2 | + | 2749 | 0.74 | 0.321286 |
Target: 5'- aGCCGaGAUCGCGGcCGCcGug--CCGGu -3' miRNA: 3'- -CGGC-CUAGCGCCaGCGuCuuaaGGCC- -5' |
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17178 | 3' | -54.9 | NC_004333.2 | + | 28658 | 1.12 | 0.000687 |
Target: 5'- cGCCGGAUCGCGGUCGCAGAAUUCCGGu -3' miRNA: 3'- -CGGCCUAGCGCCAGCGUCUUAAGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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