Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17178 | 3' | -54.9 | NC_004333.2 | + | 30464 | 0.66 | 0.720185 |
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Target: 5'- uCCGGcgC-CGGUCGCuucGAcgUUCGGc -3' miRNA: 3'- cGGCCuaGcGCCAGCGu--CUuaAGGCC- -5' |
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| 17178 | 3' | -54.9 | NC_004333.2 | + | 22972 | 0.66 | 0.734935 |
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Target: 5'- cGCCGGcagcGUCGCGGUaccggucgccgagaGCAGGAag-CGGa -3' miRNA: 3'- -CGGCC----UAGCGCCAg-------------CGUCUUaagGCC- -5' |
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| 17178 | 3' | -54.9 | NC_004333.2 | + | 26054 | 0.66 | 0.751546 |
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Target: 5'- cGCCGGAUUugccaGUGGcgcCGCAGcggcggcgcUUCCGGa -3' miRNA: 3'- -CGGCCUAG-----CGCCa--GCGUCuu-------AAGGCC- -5' |
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| 17178 | 3' | -54.9 | NC_004333.2 | + | 40755 | 0.66 | 0.761775 |
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Target: 5'- gGuuGGuguucuUUGCGGUCGC-GAGUgcgCCGa -3' miRNA: 3'- -CggCCu-----AGCGCCAGCGuCUUAa--GGCc -5' |
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| 17178 | 3' | -54.9 | NC_004333.2 | + | 18300 | 0.66 | 0.761775 |
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Target: 5'- uGCCG--UCG-GcGUCGUAGAuUUCCGGc -3' miRNA: 3'- -CGGCcuAGCgC-CAGCGUCUuAAGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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