Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17184 | 3' | -59.7 | NC_004333.2 | + | 31080 | 0.67 | 0.420619 |
Target: 5'- gCGAGCGCCU-GCaGGUUCggcgCCUggcCCGu -3' miRNA: 3'- aGCUCGCGGAcCG-CCAAGa---GGA---GGCu -5' |
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17184 | 3' | -59.7 | NC_004333.2 | + | 18561 | 0.67 | 0.393432 |
Target: 5'- gUCGGcGUGCCggcaacGGCGGguacgCCUCCGGu -3' miRNA: 3'- -AGCU-CGCGGa-----CCGCCaaga-GGAGGCU- -5' |
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17184 | 3' | -59.7 | NC_004333.2 | + | 46283 | 0.73 | 0.164385 |
Target: 5'- uUCGAGCGCCUGGCGcugaUCCggCgCGAg -3' miRNA: 3'- -AGCUCGCGGACCGCcaagAGGa-G-GCU- -5' |
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17184 | 3' | -59.7 | NC_004333.2 | + | 30855 | 1.08 | 0.000364 |
Target: 5'- gUCGAGCGCCUGGCGGUUCUCCUCCGAc -3' miRNA: 3'- -AGCUCGCGGACCGCCAAGAGGAGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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