miRNA: 3'- guaGCUGACCAaCU---UGCGCAGCuCGC- -5'
miRNA: 3'- gUAGCuGACCAACUUGCGcAGCuCGc -5'
miRNA: 3'- gUAGCUGac------CAaCUUGCGCAGCUCGC- -5'
miRNA: 3'- -GUAGCUGACCAACUUGCGCAGCUCGC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17184 | 5' | -52.4 | NC_004333.2 | + | 37638 | 0.75 | 0.350776 |
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Target: 5'- --aCGGCUGGUaGAccaACGCGUCG-GCGa -3' miRNA: 3'- guaGCUGACCAaCU---UGCGCAGCuCGC- -5' |
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| 17184 | 5' | -52.4 | NC_004333.2 | + | 3711 | 0.76 | 0.317693 |
|
Target: 5'- gAUCGuCUGGUUGAGCGCcUCGcGCa -3' miRNA: 3'- gUAGCuGACCAACUUGCGcAGCuCGc -5' |
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| 17184 | 5' | -52.4 | NC_004333.2 | + | 30831 | 0.76 | 0.309798 |
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Target: 5'- gGUCGACaccgcgucGUaGAACGCGUCGAGCGc -3' miRNA: 3'- gUAGCUGac------CAaCUUGCGCAGCUCGC- -5' |
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| 17184 | 5' | -52.4 | NC_004333.2 | + | 30890 | 1.08 | 0.002183 |
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Target: 5'- gCAUCGACUGGUUGAACGCGUCGAGCGc -3' miRNA: 3'- -GUAGCUGACCAACUUGCGCAGCUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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