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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17193 | 3' | -54.8 | NC_004333.2 | + | 41472 | 0.66 | 0.653996 |
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Target: 5'- aUCcAUGGCgaGCUCGAgaaGUCGGa-- -3' miRNA: 3'- gAGuUACCGgaCGAGCUa--CAGCCguu -5' |
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| 17193 | 3' | -54.8 | NC_004333.2 | + | 25716 | 0.66 | 0.641507 |
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Target: 5'- --gAAUGGCUUGCUCGccggccagcugcaGgccgGUCGGCGu -3' miRNA: 3'- gagUUACCGGACGAGC-------------Ua---CAGCCGUu -5' |
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| 17193 | 3' | -54.8 | NC_004333.2 | + | 45552 | 0.67 | 0.563479 |
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Target: 5'- uCUCGcUGaCCUGCUCGGg--CGGCAGg -3' miRNA: 3'- -GAGUuACcGGACGAGCUacaGCCGUU- -5' |
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| 17193 | 3' | -54.8 | NC_004333.2 | + | 22209 | 0.68 | 0.552332 |
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Target: 5'- -----cGGCCUGCUCGAUGcCGagacgggccGCAAa -3' miRNA: 3'- gaguuaCCGGACGAGCUACaGC---------CGUU- -5' |
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| 17193 | 3' | -54.8 | NC_004333.2 | + | 12510 | 0.68 | 0.552332 |
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Target: 5'- -aUAcgGGCCguccgGCUCGGUGUUcaGGCGu -3' miRNA: 3'- gaGUuaCCGGa----CGAGCUACAG--CCGUu -5' |
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| 17193 | 3' | -54.8 | NC_004333.2 | + | 32853 | 0.73 | 0.265423 |
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Target: 5'- -----cGGCCUGCUCGA-GUCGGuCGAu -3' miRNA: 3'- gaguuaCCGGACGAGCUaCAGCC-GUU- -5' |
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| 17193 | 3' | -54.8 | NC_004333.2 | + | 37710 | 1.06 | 0.00115 |
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Target: 5'- gCUCAAUGGCCUGCUCGAUGUCGGCAAg -3' miRNA: 3'- -GAGUUACCGGACGAGCUACAGCCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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