Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17194 | 5' | -61.5 | NC_004333.2 | + | 18503 | 0.69 | 0.272988 |
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Target: 5'- aCGCGcUCgCGGugUCGAcGCCgccgaucgCGAGGCg -3' miRNA: 3'- aGCGC-AG-GCCugAGCU-CGG--------GCUCCGg -5' |
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| 17194 | 5' | -61.5 | NC_004333.2 | + | 37938 | 0.69 | 0.247495 |
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Target: 5'- -aGCGcucaCCGGcCUCGGGCUCGAGuCCg -3' miRNA: 3'- agCGCa---GGCCuGAGCUCGGGCUCcGG- -5' |
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| 17194 | 5' | -61.5 | NC_004333.2 | + | 41434 | 0.7 | 0.229694 |
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Target: 5'- cUUGCGgUCGGGCgCGuGCUCGAGcGCCa -3' miRNA: 3'- -AGCGCaGGCCUGaGCuCGGGCUC-CGG- -5' |
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| 17194 | 5' | -61.5 | NC_004333.2 | + | 45787 | 0.71 | 0.182693 |
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Target: 5'- uUCGCG-CCGGGCUCGAGa-UGcAGGCg -3' miRNA: 3'- -AGCGCaGGCCUGAGCUCggGC-UCCGg -5' |
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| 17194 | 5' | -61.5 | NC_004333.2 | + | 12955 | 0.73 | 0.136951 |
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Target: 5'- cCGCuaUCCGGGCUCGAcgcacugcagGCCC-AGGCCc -3' miRNA: 3'- aGCGc-AGGCCUGAGCU----------CGGGcUCCGG- -5' |
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| 17194 | 5' | -61.5 | NC_004333.2 | + | 10147 | 0.66 | 0.422453 |
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Target: 5'- cCGCGaUCC-GAC-CGAGCUCG-GGCa -3' miRNA: 3'- aGCGC-AGGcCUGaGCUCGGGCuCCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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