Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17194 | 5' | -61.5 | NC_004333.2 | + | 41434 | 0.7 | 0.229694 |
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Target: 5'- cUUGCGgUCGGGCgCGuGCUCGAGcGCCa -3' miRNA: 3'- -AGCGCaGGCCUGaGCuCGGGCUC-CGG- -5' |
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| 17194 | 5' | -61.5 | NC_004333.2 | + | 26085 | 0.7 | 0.224005 |
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Target: 5'- gCGCuUCCGGAUUCGGGCaggcgacuUCGGGGUa -3' miRNA: 3'- aGCGcAGGCCUGAGCUCG--------GGCUCCGg -5' |
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| 17194 | 5' | -61.5 | NC_004333.2 | + | 19938 | 0.71 | 0.202435 |
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Target: 5'- gCGCG-CCGGGCggCGAGCCgccgCGAGuGCUu -3' miRNA: 3'- aGCGCaGGCCUGa-GCUCGG----GCUC-CGG- -5' |
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| 17194 | 5' | -61.5 | NC_004333.2 | + | 45787 | 0.71 | 0.182693 |
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Target: 5'- uUCGCG-CCGGGCUCGAGa-UGcAGGCg -3' miRNA: 3'- -AGCGCaGGCCUGAGCUCggGC-UCCGg -5' |
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| 17194 | 5' | -61.5 | NC_004333.2 | + | 12955 | 0.73 | 0.136951 |
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Target: 5'- cCGCuaUCCGGGCUCGAcgcacugcagGCCC-AGGCCc -3' miRNA: 3'- aGCGc-AGGCCUGAGCU----------CGGGcUCCGG- -5' |
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| 17194 | 5' | -61.5 | NC_004333.2 | + | 37855 | 1.12 | 0.000158 |
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Target: 5'- aUCGCGUCCGGACUCGAGCCCGAGGCCg -3' miRNA: 3'- -AGCGCAGGCCUGAGCUCGGGCUCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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