miRNA: 3'- -GCUCCUAgUGCGGCACua-GCG-UGUUC- -5'
miRNA: 3'- -GCUCCUA--GUGCG-GCa---CUAGCGUGUUc -5'
miRNA: 3'- -GCUCC-UA-GUGCGGCACUa--GCGUGUUC- -5'
miRNA: 3'- -GCUCCUAGUGCGGCACUAGCGUGUUC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17216 | 5' | -53.2 | NC_004333.2 | + | 42081 | 0.74 | 0.366166 |
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Target: 5'- aCGAGGAUaGCGUCGUGcugCGCgACGAGg -3' miRNA: 3'- -GCUCCUAgUGCGGCACua-GCG-UGUUC- -5' |
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| 17216 | 5' | -53.2 | NC_004333.2 | + | 29974 | 0.75 | 0.315991 |
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Target: 5'- cCGAGGAUgcCGCGCgCGgcccGAUCGCGCAGc -3' miRNA: 3'- -GCUCCUA--GUGCG-GCa---CUAGCGUGUUc -5' |
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| 17216 | 5' | -53.2 | NC_004333.2 | + | 40105 | 0.77 | 0.225524 |
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Target: 5'- gCGAGGcGUcCGCGCCGUGGcgcCGCGCGAGu -3' miRNA: 3'- -GCUCC-UA-GUGCGGCACUa--GCGUGUUC- -5' |
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| 17216 | 5' | -53.2 | NC_004333.2 | + | 46139 | 1.1 | 0.001312 |
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Target: 5'- uCGAGGAUCACGCCGUGAUCGCACAAGg -3' miRNA: 3'- -GCUCCUAGUGCGGCACUAGCGUGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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