Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17224 | 5' | -45.5 | NC_004348.1 | + | 28425 | 0.66 | 0.984935 |
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Target: 5'- uUAGCAGCAUUgaaagcggcaagcgcACGGCUGuuguCAUAGUCAu -3' miRNA: 3'- -GUUGUCGUAA---------------UGUUGACc---GUGUUAGU- -5' |
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| 17224 | 5' | -45.5 | NC_004348.1 | + | 36606 | 0.67 | 0.973277 |
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Target: 5'- uCAGCAGCAUUagcgaacugaAUAGCcugcugcgcuucUGGUGCGAUCGu -3' miRNA: 3'- -GUUGUCGUAA----------UGUUG------------ACCGUGUUAGU- -5' |
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| 17224 | 5' | -45.5 | NC_004348.1 | + | 10235 | 0.68 | 0.962259 |
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Target: 5'- uGACAGag--GCAauguACUGGCGCAaGUCAg -3' miRNA: 3'- gUUGUCguaaUGU----UGACCGUGU-UAGU- -5' |
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| 17224 | 5' | -45.5 | NC_004348.1 | + | 18449 | 0.68 | 0.953363 |
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Target: 5'- uGGCAGCGggaaaUUACcuACUGGCGacCGAUCAc -3' miRNA: 3'- gUUGUCGU-----AAUGu-UGACCGU--GUUAGU- -5' |
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| 17224 | 5' | -45.5 | NC_004348.1 | + | 8359 | 0.71 | 0.862765 |
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Target: 5'- gCGGCGGCGagGCGGCUggcGGCAUAGUUg -3' miRNA: 3'- -GUUGUCGUaaUGUUGA---CCGUGUUAGu -5' |
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| 17224 | 5' | -45.5 | NC_004348.1 | + | 22141 | 1.1 | 0.0052 |
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Target: 5'- gCAACAGCAUUACAACUGGCACAAUCAg -3' miRNA: 3'- -GUUGUCGUAAUGUUGACCGUGUUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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