Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17228 | 5' | -53.7 | NC_004348.1 | + | 32175 | 1.09 | 0.000931 |
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Target: 5'- aAUCGAACCUCAACGCCUCCAUCACCCc -3' miRNA: 3'- -UAGCUUGGAGUUGCGGAGGUAGUGGG- -5' |
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| 17228 | 5' | -53.7 | NC_004348.1 | + | 7788 | 0.74 | 0.263168 |
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Target: 5'- gGUUGAcCUUCcGCGCCUCCAguucugcCACCCg -3' miRNA: 3'- -UAGCUuGGAGuUGCGGAGGUa------GUGGG- -5' |
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| 17228 | 5' | -53.7 | NC_004348.1 | + | 24179 | 0.68 | 0.594694 |
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Target: 5'- -cUGAucuUCUCAACGCUaacaUCAUCGCCCg -3' miRNA: 3'- uaGCUu--GGAGUUGCGGa---GGUAGUGGG- -5' |
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| 17228 | 5' | -53.7 | NC_004348.1 | + | 10394 | 0.68 | 0.606048 |
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Target: 5'- uAUCG-ACCa-GACGUUUCCAUCcgGCCCg -3' miRNA: 3'- -UAGCuUGGagUUGCGGAGGUAG--UGGG- -5' |
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| 17228 | 5' | -53.7 | NC_004348.1 | + | 26376 | 0.67 | 0.617427 |
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Target: 5'- -aCG-ACCUCGAUGCUgaugauauUCCAUCAUUCc -3' miRNA: 3'- uaGCuUGGAGUUGCGG--------AGGUAGUGGG- -5' |
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| 17228 | 5' | -53.7 | NC_004348.1 | + | 34495 | 0.66 | 0.71911 |
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Target: 5'- -gCGAACCagAACGCUcccgggcagCCAUCACUa -3' miRNA: 3'- uaGCUUGGagUUGCGGa--------GGUAGUGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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