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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17230 | 3' | -59.6 | NC_004348.1 | + | 2693 | 0.66 | 0.366474 |
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Target: 5'- -aGGCGCUGGCGAacuuuuccgguuUUGuUAUC-GCCGUa -3' miRNA: 3'- ggCCGCGACCGCU------------GAC-GUAGuCGGUA- -5' |
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| 17230 | 3' | -59.6 | NC_004348.1 | + | 6230 | 0.66 | 0.35785 |
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Target: 5'- aCGGCGagccGCGGCUGCAUaucaaagagCAGCCu- -3' miRNA: 3'- gGCCGCgac-CGCUGACGUA---------GUCGGua -5' |
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| 17230 | 3' | -59.6 | NC_004348.1 | + | 31874 | 0.67 | 0.341034 |
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Target: 5'- -aGGCGCaaccaccguUGGCG-CUGCGUuguaugucgcgCAGCCAUc -3' miRNA: 3'- ggCCGCG---------ACCGCuGACGUA-----------GUCGGUA- -5' |
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| 17230 | 3' | -59.6 | NC_004348.1 | + | 33614 | 0.69 | 0.245832 |
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Target: 5'- gUGGUGUUGGCGAgaaUGUAUC-GCCAg -3' miRNA: 3'- gGCCGCGACCGCUg--ACGUAGuCGGUa -5' |
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| 17230 | 3' | -59.6 | NC_004348.1 | + | 36166 | 1.07 | 0.000325 |
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Target: 5'- cCCGGCGCUGGCGACUGCAUCAGCCAUu -3' miRNA: 3'- -GGCCGCGACCGCUGACGUAGUCGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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