Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17230 | 5' | -46.2 | NC_004348.1 | + | 19458 | 0.66 | 0.98615 |
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Target: 5'- ----cUUUGCgUAGAUUAGCcauuuGCCAGCUc -3' miRNA: 3'- ccuauAAACG-GUCUAAUUG-----CGGUCGG- -5' |
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| 17230 | 5' | -46.2 | NC_004348.1 | + | 2041 | 0.67 | 0.975445 |
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Target: 5'- -------aGCCAGcaccauauccGCGCCAGCCu -3' miRNA: 3'- ccuauaaaCGGUCuaau------UGCGGUCGG- -5' |
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| 17230 | 5' | -46.2 | NC_004348.1 | + | 26609 | 0.67 | 0.970346 |
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Target: 5'- -------cGCCAGAUUAAgCGUC-GCCg -3' miRNA: 3'- ccuauaaaCGGUCUAAUU-GCGGuCGG- -5' |
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| 17230 | 5' | -46.2 | NC_004348.1 | + | 37008 | 0.67 | 0.966798 |
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Target: 5'- aGGAUGgaUGUCAGAggagaaagGAcacaaaugccacCGCCAGCUg -3' miRNA: 3'- -CCUAUaaACGGUCUaa------UU------------GCGGUCGG- -5' |
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| 17230 | 5' | -46.2 | NC_004348.1 | + | 8969 | 0.68 | 0.96296 |
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Target: 5'- cGGAa--UUGCCGGAaagcGGCGCgGGUCa -3' miRNA: 3'- -CCUauaAACGGUCUaa--UUGCGgUCGG- -5' |
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| 17230 | 5' | -46.2 | NC_004348.1 | + | 2097 | 0.68 | 0.96296 |
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Target: 5'- cGGAUAUggUGCUGGcuaAGCGCuCAGaCCg -3' miRNA: 3'- -CCUAUAa-ACGGUCuaaUUGCG-GUC-GG- -5' |
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| 17230 | 5' | -46.2 | NC_004348.1 | + | 33593 | 0.7 | 0.914276 |
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Target: 5'- -------cGCCAGA---ACGUCAGCCc -3' miRNA: 3'- ccuauaaaCGGUCUaauUGCGGUCGG- -5' |
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| 17230 | 5' | -46.2 | NC_004348.1 | + | 28298 | 0.72 | 0.830062 |
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Target: 5'- cGGAUAUUaaugccuaucUGUCAGAagAAgGCCuGGCCa -3' miRNA: 3'- -CCUAUAA----------ACGGUCUaaUUgCGG-UCGG- -5' |
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| 17230 | 5' | -46.2 | NC_004348.1 | + | 22178 | 0.72 | 0.830062 |
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Target: 5'- uGAUAUUUGCCgugauAGAUguAUGCCGuuGCCg -3' miRNA: 3'- cCUAUAAACGG-----UCUAauUGCGGU--CGG- -5' |
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| 17230 | 5' | -46.2 | NC_004348.1 | + | 36132 | 1.16 | 0.002124 |
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Target: 5'- gGGAUAUUUGCCAGAUUAACGCCAGCCu -3' miRNA: 3'- -CCUAUAAACGGUCUAAUUGCGGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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