Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17232 | 3' | -53.1 | NC_004348.1 | + | 31910 | 0.66 | 0.762209 |
Target: 5'- gCAGcCAUCguuaaUGGUACagaaGGGAUUGCCGGa -3' miRNA: 3'- -GUC-GUGGag---GCUAUG----CCUUAACGGCC- -5' |
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17232 | 3' | -53.1 | NC_004348.1 | + | 7974 | 0.66 | 0.740871 |
Target: 5'- gCAGCGCCagCUGGUgGCGGggUUGgCu- -3' miRNA: 3'- -GUCGUGGa-GGCUA-UGCCuuAACgGcc -5' |
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17232 | 3' | -53.1 | NC_004348.1 | + | 33924 | 0.67 | 0.685706 |
Target: 5'- uGGCACCUUCGccgccGCaGGuUUGCCGGc -3' miRNA: 3'- gUCGUGGAGGCua---UGcCUuAACGGCC- -5' |
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17232 | 3' | -53.1 | NC_004348.1 | + | 9019 | 0.69 | 0.606494 |
Target: 5'- cCGGCAaUUCCGuauCGGAggUGCUGGa -3' miRNA: 3'- -GUCGUgGAGGCuauGCCUuaACGGCC- -5' |
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17232 | 3' | -53.1 | NC_004348.1 | + | 9059 | 0.72 | 0.434115 |
Target: 5'- cCAGCGCCUCUauauGGUGCGGAugacccgcGCCGc -3' miRNA: 3'- -GUCGUGGAGG----CUAUGCCUuaa-----CGGCc -5' |
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17232 | 3' | -53.1 | NC_004348.1 | + | 5753 | 0.72 | 0.414508 |
Target: 5'- uGGCACuCUCCGGUAauucCGGGug-GCUGGa -3' miRNA: 3'- gUCGUG-GAGGCUAU----GCCUuaaCGGCC- -5' |
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17232 | 3' | -53.1 | NC_004348.1 | + | 5880 | 0.73 | 0.376975 |
Target: 5'- uCAGCACUUgUGAUGCuGA--UGCCGGg -3' miRNA: 3'- -GUCGUGGAgGCUAUGcCUuaACGGCC- -5' |
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17232 | 3' | -53.1 | NC_004348.1 | + | 8954 | 1.11 | 0.000875 |
Target: 5'- cCAGCACCUCCGAUACGGAAUUGCCGGa -3' miRNA: 3'- -GUCGUGGAGGCUAUGCCUUAACGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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