Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17234 | 5' | -51.5 | NC_004348.1 | + | 37689 | 0.66 | 0.798888 |
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Target: 5'- gGCGAGCau-UGCaUUACGUGCUGGu-- -3' miRNA: 3'- -CGUUCGguuGCG-GAUGUACGACCuuc -5' |
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| 17234 | 5' | -51.5 | NC_004348.1 | + | 33373 | 0.67 | 0.756483 |
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Target: 5'- uCAGGUCGAUG-UUGCGUGUUGGAGu -3' miRNA: 3'- cGUUCGGUUGCgGAUGUACGACCUUc -5' |
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| 17234 | 5' | -51.5 | NC_004348.1 | + | 18985 | 0.67 | 0.749903 |
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Target: 5'- aGCGGGCUuuaacguGCGCuaaCUGCGgucagaagcugcaugUGCUGGAAGu -3' miRNA: 3'- -CGUUCGGu------UGCG---GAUGU---------------ACGACCUUC- -5' |
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| 17234 | 5' | -51.5 | NC_004348.1 | + | 6131 | 0.67 | 0.734367 |
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Target: 5'- cGCAAgGCUAuaACGCCUGCcgcaaugccAUGCUcaacGGAGGu -3' miRNA: 3'- -CGUU-CGGU--UGCGGAUG---------UACGA----CCUUC- -5' |
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| 17234 | 5' | -51.5 | NC_004348.1 | + | 2109 | 0.68 | 0.711788 |
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Target: 5'- gGCAGGCUGGCGCggAUaugGUGCUGGcuAAGc -3' miRNA: 3'- -CGUUCGGUUGCGgaUG---UACGACC--UUC- -5' |
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| 17234 | 5' | -51.5 | NC_004348.1 | + | 36108 | 0.74 | 0.353528 |
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Target: 5'- uGCAGucGCCAGCGCCgggGCAUggGCUGGucAGa -3' miRNA: 3'- -CGUU--CGGUUGCGGa--UGUA--CGACCu-UC- -5' |
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| 17234 | 5' | -51.5 | NC_004348.1 | + | 8045 | 0.75 | 0.294802 |
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Target: 5'- cGCAAGCCAAcccCGCCacCAUGCUGGc-- -3' miRNA: 3'- -CGUUCGGUU---GCGGauGUACGACCuuc -5' |
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| 17234 | 5' | -51.5 | NC_004348.1 | + | 26996 | 1.11 | 0.001045 |
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Target: 5'- cGCAAGCCAACGCCUACAUGCUGGAAGc -3' miRNA: 3'- -CGUUCGGUUGCGGAUGUACGACCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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