miRNA: 3'- guuCGCGUCa----GU--CGCGUCa---UAaGGUG- -5'
miRNA: 3'- guuCGCGUCa----GU--CGCGUCa---UAaGGUG- -5'
miRNA: 3'- gUUcGCGUCAGUCGCGU-CAuaa---GGUG- -5'
miRNA: 3'- gUUCGCguCaGUCgCGUCA----UAAGgUG- -5'
miRNA: 3'- -GUUCGCGUCAGUCGCGUCAUAAGGUG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17236 | 3' | -52.4 | NC_004348.1 | + | 11374 | 0.66 | 0.769083 |
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Target: 5'- --cGCGCAGgucggCAccGCGCAGguugGUaCCGCa -3' miRNA: 3'- guuCGCGUCa----GU--CGCGUCa---UAaGGUG- -5' |
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| 17236 | 3' | -52.4 | NC_004348.1 | + | 11509 | 0.66 | 0.769083 |
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Target: 5'- --cGCGCAGgucggCAccGCGCAGguugGUaCCGCa -3' miRNA: 3'- guuCGCGUCa----GU--CGCGUCa---UAaGGUG- -5' |
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| 17236 | 3' | -52.4 | NC_004348.1 | + | 19055 | 0.66 | 0.769083 |
|
Target: 5'- uGAcCGCAGUUAGCGCAcGUuaaagcCCGCu -3' miRNA: 3'- gUUcGCGUCAGUCGCGU-CAuaa---GGUG- -5' |
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| 17236 | 3' | -52.4 | NC_004348.1 | + | 32306 | 0.67 | 0.679246 |
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Target: 5'- gAAGCGguGaCAGgGCAGUugcaAUUCgACa -3' miRNA: 3'- gUUCGCguCaGUCgCGUCA----UAAGgUG- -5' |
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| 17236 | 3' | -52.4 | NC_004348.1 | + | 31007 | 1.1 | 0.000873 |
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Target: 5'- uCAAGCGCAGUCAGCGCAGUAUUCCACa -3' miRNA: 3'- -GUUCGCGUCAGUCGCGUCAUAAGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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