Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17236 | 5' | -49 | NC_004348.1 | + | 28663 | 0.66 | 0.931495 |
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Target: 5'- -gGCGGAcUU-CUCCGGGaacagGCGCa -3' miRNA: 3'- gaCGUCUuAAcGAGGCCUaaua-CGCG- -5' |
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| 17236 | 5' | -49 | NC_004348.1 | + | 35075 | 0.66 | 0.919024 |
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Target: 5'- cCUGCuuuAUUGCUgCGGAUUGUuucaGCa -3' miRNA: 3'- -GACGucuUAACGAgGCCUAAUAcg--CG- -5' |
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| 17236 | 5' | -49 | NC_004348.1 | + | 30219 | 0.66 | 0.919024 |
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Target: 5'- -gGCAGAccaacaccgAUUGCuUCCGGuc-GUGuCGCg -3' miRNA: 3'- gaCGUCU---------UAACG-AGGCCuaaUAC-GCG- -5' |
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| 17236 | 5' | -49 | NC_004348.1 | + | 19026 | 0.67 | 0.865504 |
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Target: 5'- gUGCuGGAAguucacgugUGCUCCG-AUUGcUGCGCa -3' miRNA: 3'- gACG-UCUUa--------ACGAGGCcUAAU-ACGCG- -5' |
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| 17236 | 5' | -49 | NC_004348.1 | + | 15384 | 0.71 | 0.686615 |
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Target: 5'- uUGguGGAUUGCuUCUGGAUgAUGacaGCa -3' miRNA: 3'- gACguCUUAACG-AGGCCUAaUACg--CG- -5' |
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| 17236 | 5' | -49 | NC_004348.1 | + | 31041 | 1.13 | 0.001475 |
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Target: 5'- cCUGCAGAAUUGCUCCGGAUUAUGCGCu -3' miRNA: 3'- -GACGUCUUAACGAGGCCUAAUACGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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