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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17268 | 3' | -62.8 | NC_004366.1 | + | 1432 | 0.66 | 0.031542 |
Target: 5'- aGCCGCGGGAuCCaagguucaaUGCCUGCUuUGCc -3' miRNA: 3'- gCGGCGCCCUcGGga-------GCGGGUGA-ACG- -5' |
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17268 | 3' | -62.8 | NC_004366.1 | + | 3458 | 0.71 | 0.009778 |
Target: 5'- gGUgGUGGGAGCCCggUCGCagUugUUGCa -3' miRNA: 3'- gCGgCGCCCUCGGG--AGCGg-GugAACG- -5' |
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17268 | 3' | -62.8 | NC_004366.1 | + | 665 | 1.11 | 0.000002 |
Target: 5'- cCGCCGCGGGAGCCCUCGCCCACUUGCa -3' miRNA: 3'- -GCGGCGCCCUCGGGAGCGGGUGAACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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