Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17273 | 3' | -60.7 | NC_004367.1 | + | 129845 | 0.67 | 0.657348 |
Target: 5'- gUCCCCGaaacCCCcaagGGGCGGccgGGUCCCUa -3' miRNA: 3'- -AGGGGCa---GGGcg--CCUGUCa--UCAGGGG- -5' |
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17273 | 3' | -60.7 | NC_004367.1 | + | 126936 | 0.67 | 0.657348 |
Target: 5'- gUCCCCGaaacCCCcaagGGGCGGccgGGUCCCUa -3' miRNA: 3'- -AGGGGCa---GGGcg--CCUGUCa--UCAGGGG- -5' |
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17273 | 3' | -60.7 | NC_004367.1 | + | 124027 | 0.67 | 0.657348 |
Target: 5'- gUCCCCGaaacCCCcaagGGGCGGccgGGUCCCUa -3' miRNA: 3'- -AGGGGCa---GGGcg--CCUGUCa--UCAGGGG- -5' |
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17273 | 3' | -60.7 | NC_004367.1 | + | 121118 | 0.67 | 0.657348 |
Target: 5'- gUCCCCGaaacCCCcaagGGGCGGccgGGUCCCUa -3' miRNA: 3'- -AGGGGCa---GGGcg--CCUGUCa--UCAGGGG- -5' |
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17273 | 3' | -60.7 | NC_004367.1 | + | 115739 | 0.91 | 0.023551 |
Target: 5'- gUCCCCGUCUCGCGGACAacuGUCCCCg -3' miRNA: 3'- -AGGGGCAGGGCGCCUGUcauCAGGGG- -5' |
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17273 | 3' | -60.7 | NC_004367.1 | + | 115697 | 0.96 | 0.010669 |
Target: 5'- gUCCCCGUCCCGCGGACAacuGUCCCCg -3' miRNA: 3'- -AGGGGCAGGGCGCCUGUcauCAGGGG- -5' |
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17273 | 3' | -60.7 | NC_004367.1 | + | 115655 | 0.78 | 0.178929 |
Target: 5'- gUCCCCGUCCUGCGGACugagggcagcuguccAcGUAccaugcccacucGUCCCCa -3' miRNA: 3'- -AGGGGCAGGGCGCCUG---------------U-CAU------------CAGGGG- -5' |
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17273 | 3' | -60.7 | NC_004367.1 | + | 115399 | 0.68 | 0.618214 |
Target: 5'- gUCCUCagaCCGCGGACAauaAGUCCCa -3' miRNA: 3'- -AGGGGcagGGCGCCUGUca-UCAGGGg -5' |
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17273 | 3' | -60.7 | NC_004367.1 | + | 56479 | 0.73 | 0.329767 |
Target: 5'- gCUCCGcCCUGUGGGuuGUAGUCCCa -3' miRNA: 3'- aGGGGCaGGGCGCCUguCAUCAGGGg -5' |
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17273 | 3' | -60.7 | NC_004367.1 | + | 56253 | 0.82 | 0.090708 |
Target: 5'- gUCCCCGUCCCGCGGACAuuug-CCCa -3' miRNA: 3'- -AGGGGCAGGGCGCCUGUcaucaGGGg -5' |
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17273 | 3' | -60.7 | NC_004367.1 | + | 56044 | 0.94 | 0.014272 |
Target: 5'- gUCCCCGUCCCGCGGACAGUuguacccGUCCCg -3' miRNA: 3'- -AGGGGCAGGGCGCCUGUCAu------CAGGGg -5' |
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17273 | 3' | -60.7 | NC_004367.1 | + | 56002 | 1.04 | 0.002685 |
Target: 5'- gUCCCCGUCCCGCGGACAGUuGUCCCCg -3' miRNA: 3'- -AGGGGCAGGGCGCCUGUCAuCAGGGG- -5' |
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17273 | 3' | -60.7 | NC_004367.1 | + | 55859 | 0.7 | 0.522832 |
Target: 5'- gUCCgCGggacggguacaacugUCCGCGGGCGGggacguGUCCCCc -3' miRNA: 3'- -AGGgGCa--------------GGGCGCCUGUCau----CAGGGG- -5' |
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17273 | 3' | -60.7 | NC_004367.1 | + | 26739 | 0.69 | 0.569555 |
Target: 5'- --aCCGcCCCGCGGACAGg---CCaCCa -3' miRNA: 3'- aggGGCaGGGCGCCUGUCaucaGG-GG- -5' |
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17273 | 3' | -60.7 | NC_004367.1 | + | 22682 | 0.82 | 0.090708 |
Target: 5'- gUCCCCGUCCCGCGGACAuuug-CCCa -3' miRNA: 3'- -AGGGGCAGGGCGCCUGUcaucaGGGg -5' |
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17273 | 3' | -60.7 | NC_004367.1 | + | 22473 | 1.12 | 0.000811 |
Target: 5'- gUCCCCGUCCCGCGGACAGUAGUCCCCg -3' miRNA: 3'- -AGGGGCAGGGCGCCUGUCAUCAGGGG- -5' |
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17273 | 3' | -60.7 | NC_004367.1 | + | 22431 | 1.12 | 0.000811 |
Target: 5'- gUCCCCGUCCCGCGGACAGUAGUCCCCg -3' miRNA: 3'- -AGGGGCAGGGCGCCUGUCAUCAGGGG- -5' |
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17273 | 3' | -60.7 | NC_004367.1 | + | 22406 | 0.86 | 0.051574 |
Target: 5'- ---nCGUCCCGCGGACAGUuGUCCCCg -3' miRNA: 3'- agggGCAGGGCGCCUGUCAuCAGGGG- -5' |
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17273 | 3' | -60.7 | NC_004367.1 | + | 22036 | 0.68 | 0.641704 |
Target: 5'- gCUCCGgagccugccUCCCGCGGGCuucuuugcagggcuGUAGggguuUCCCCg -3' miRNA: 3'- aGGGGC---------AGGGCGCCUGu-------------CAUC-----AGGGG- -5' |
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17273 | 3' | -60.7 | NC_004367.1 | + | 12902 | 0.68 | 0.627021 |
Target: 5'- aUCUUCGUCCCGUugugaaaacacagGGGCGGcucUGGaCCCCu -3' miRNA: 3'- -AGGGGCAGGGCG-------------CCUGUC---AUCaGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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