miRNA: 3'- auaaGGCCAG----UGaCGGCCCGaa--GGUC- -5'
miRNA: 3'- auaAGGccAGUGACGGCCCGaa-----GGUc -5'
miRNA: 3'- auAAGGCCa----GU---GAcGGCCCGAAGGUC- -5'
miRNA: 3'- auAAGGCCa----GU---GAcGGCCCGAAGGUC- -5'
miRNA: 3'- -AUAAGGCCAGUGACGGCCCGAAGGUC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17275 | 3' | -56.7 | NC_004367.1 | + | 5704 | 0.66 | 0.873002 |
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Target: 5'- ---aCCGGUCuuuaGCgGCUGGGCgggcCCGGa -3' miRNA: 3'- auaaGGCCAG----UGaCGGCCCGaa--GGUC- -5' |
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| 17275 | 3' | -56.7 | NC_004367.1 | + | 79355 | 0.66 | 0.873002 |
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Target: 5'- --aUCC--UCACUGCCGGGCgugugacCCAa -3' miRNA: 3'- auaAGGccAGUGACGGCCCGaa-----GGUc -5' |
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| 17275 | 3' | -56.7 | NC_004367.1 | + | 116726 | 0.66 | 0.857899 |
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Target: 5'- -uUUCCGGgugccCAggcCUcCCGGGUUUCCGGg -3' miRNA: 3'- auAAGGCCa----GU---GAcGGCCCGAAGGUC- -5' |
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| 17275 | 3' | -56.7 | NC_004367.1 | + | 116879 | 0.66 | 0.857899 |
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Target: 5'- -uUUCCGGgugccCAggcCUcCCGGGUUUCCGGg -3' miRNA: 3'- auAAGGCCa----GU---GAcGGCCCGAAGGUC- -5' |
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| 17275 | 3' | -56.7 | NC_004367.1 | + | 26511 | 1.08 | 0.00256 |
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Target: 5'- aUAUUCCGGUCACUGCCGGGCUUCCAGg -3' miRNA: 3'- -AUAAGGCCAGUGACGGCCCGAAGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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