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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17280 | 3' | -52.4 | NC_004367.1 | + | 5716 | 0.68 | 0.944204 |
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Target: 5'- --cGGCUgGGcgGGcCCggaUGUGACAGGa -3' miRNA: 3'- ugaCUGAgUCuaCCaGG---ACACUGUCC- -5' |
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| 17280 | 3' | -52.4 | NC_004367.1 | + | 120500 | 0.68 | 0.939491 |
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Target: 5'- --gGGCUCcaGGGUGGUCCUgGUGGgucuUAGGg -3' miRNA: 3'- ugaCUGAG--UCUACCAGGA-CACU----GUCC- -5' |
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| 17280 | 3' | -52.4 | NC_004367.1 | + | 123409 | 0.68 | 0.939491 |
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Target: 5'- --gGGCUCcaGGGUGGUCCUgGUGGgucuUAGGg -3' miRNA: 3'- ugaCUGAG--UCUACCAGGA-CACU----GUCC- -5' |
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| 17280 | 3' | -52.4 | NC_004367.1 | + | 126318 | 0.68 | 0.939491 |
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Target: 5'- --gGGCUCcaGGGUGGUCCUgGUGGgucuUAGGg -3' miRNA: 3'- ugaCUGAG--UCUACCAGGA-CACU----GUCC- -5' |
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| 17280 | 3' | -52.4 | NC_004367.1 | + | 129227 | 0.68 | 0.939491 |
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Target: 5'- --gGGCUCcaGGGUGGUCCUgGUGGgucuUAGGg -3' miRNA: 3'- ugaCUGAG--UCUACCAGGA-CACU----GUCC- -5' |
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| 17280 | 3' | -52.4 | NC_004367.1 | + | 53523 | 1.12 | 0.004335 |
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Target: 5'- aACUGACUCAGAUGGUCCUGUGACAGGg -3' miRNA: 3'- -UGACUGAGUCUACCAGGACACUGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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